黄曲霉毒素解毒酶对岭南黄肉仔鸡日粮中黄曲霉毒素B_1解毒效果的研究  

A Study on Antidotal Effect of Aflatoxin-detoxifizyme on Aflatoxin B_1 in Lingnan Yellow Broilers Diets

作　　者：曹红[1,2] 尹逊慧 陈善林 胡亚冬 胡熔 李世川 谢春芳[1] 苏全 姚冬生[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632 [2]广州科仁生物工程有限公司,广州510632

出　　处：《动物营养学报》2010年第2期424-430,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：十一五863重点项目(2007AA100605);广东省高校科技成果转化重大项目(cgzhzd0604);广东省产业技术研究与开发资金计划项目(0712220800003)

摘　　要：本文通过测定岭南黄肉仔鸡生长性能和血清指标及对肝脏组织切片的观察,旨在探讨黄曲霉毒素解毒酶对黄曲霉毒素B1(AFB1)的解毒作用。选用1日龄健康岭南黄肉仔鸡420羽,随机分为7个处理,每个处理4个重复,每个重复15羽,雄鸡10羽,雌鸡5羽。7个处理分别为基础日粮组、基础日粮+100μg/kg AFB1组、基础日粮+100μg/kg AFB1+0.1%解毒酶组、基础日粮+100μg/kg AFB1+0.2%解毒酶组、基础日粮+100μg/kg AFB1+0.3%解毒酶组、基础日粮+100μg/kg AFB1+0.4%解毒酶组、基础日粮+100μg/kg AFB1+0.5%解毒酶组,试验期42d。结果表明:1)基础日粮中加入100μg/kg AFB1,肉仔鸡的平均日采食量、平均日增重和平均末重有所下降(P>0.05);血清中总蛋白(P<0.05)、白蛋白(P>0.05)和球蛋白(P<0.05)含量下降,谷草转氨酶、谷丙转氨酶活性升高(P<0.05);肝脏组织病理切片观察到肝细胞广泛性坏死,胆管异常增生;2)在100μg/kg AFB1日粮中加入黄曲霉毒素解毒酶,能一定程度的改善肉仔鸡的平均日采食量、平均日增重和平均末重(P>0.05),0.2%、0.4%和0.5%的水平能显著降低毒素在肝脏和血液中的残留(P<0.05),减弱毒素对肝脏的损伤,一定程度地改善血清学指标。由此可知,黄曲霉毒素解毒酶能有效保护肉仔鸡肝脏,减轻或基本消除AFB1对肉仔鸡组织器官的不良影响,一定程度的提高肉仔鸡的平均日采食量和日增重。The experiment was conducted to determine the effects of aflatoxin-detoxifizyme on growth performance, serum and liver histological changes of Lingnan yellow broilers fed a basal diet with aflatoxin B1. Four hundred and twenty 1-dayold Lingnan yellow broiler chicks were randomly divided into 7 treatments, and 4 replicates in each treatment and 15 yellow broiler chicks including 10 roosters and 5 hens in each replicate. The 7 groups were as following： basal diet, basal diet ＋ 100 μg/kg aflatoxin B1, basal diet ＋ 100 μg/kg aflatoxin B1 ＋ 0.1% aflatoxin-detoxifizyme, basal diet ＋ 100 μg/kg aflatoxin B1 ＋ 0.2% aflatoxin-detoxifizyme, basal diet ＋ 100 μg/kg aflatoxin B1 ＋ 0.3% aflatoxin-detoxifizyme, basal diet ＋ 100 μg/kg aflatoxin B1 ＋ 0.4% aflatoxin-detoxifizyme, basal diet ＋ 100 μg/kg aflatoxin B1 ＋ 0.5% aflatoxin-detoxifizyme. The experiment lasted for 42 days. The results showed that 1） growth performance of broilers fed 100μg/kg aflatoxin B1 was affected： average daily feed intake （ADFI）, average daily gain （ADG） and average final weight （AFW） were decreased （P〉0.05） ; the contents of total protein, albumin and globulin in serum of broilers fed 100 μg/kg aflatoxin B1 were decreased （P〈0.05, P〉0.05, P〈0.05）, but activities of glutamic oxaloacetic transaminase （GOT） and glutamic pyruvic transaminase （GPT） were significantly increased （P〉0.05） ; generalized necrosis of liver and abnormal proliferation of bile duct of broilers fed 100 μg/kg aflatoxin B1 were found through liver tissue sections; 2） supplementation of aflatoxin-detoxifizyme in 100 μg/kg aflatoxin B1 diet improved ADFI, ADG and AFW of chickens to a certain degree （P〉0.05）, and 0.2%, 0.4% and 0. 5% aflatoxin-detoxifizyme significantly decreased residues of aflatoxin B1 in liver and blood （P〈0.05） and reduced the hazard of aflatoxin B1 to liver, and improved serology indexes to a certain degree. In conclusion, aflatoxin-detoxifizyme could efficiently protect th

关 键 词：黄曲霉毒素解毒酶 黄曲霉毒素 岭南黄肉仔鸡 生长性能 血清指标 

分 类 号：S831[农业科学—畜牧学]