复方浙贝颗粒联合阿霉素对P388移植瘤p53基因表达影响  

The Impact of Compound Zhe-bei Granule and Adriamycin on p53 Gene Expression in the P388 Xenograft Tumor

作　　者：陈菊[1] 孙叙敏[1] 李冬云[1] 陈信义[1] 

机构地区：[1]北京中医药大学东直门医院肿瘤血液科,100700

出　　处：《医学研究杂志》2010年第3期32-35,143,共5页Journal of Medical Research

基　　金：国家自然科学基金面上项目(30672691);教育部科技重点项目(107015)

摘　　要：目的探讨复方浙贝颗粒联合阿霉素对P388移植瘤的p53基因表达影响。方法将小鼠淋巴细胞白血病细胞系P388细胞接种于昆明小鼠腋前皮下构建小鼠移植瘤模型,予复方浙贝颗粒(灌胃)联合不同剂量的阿霉素(腹腔注射)治疗。治疗14天后处死小鼠,剥离肿瘤,荧光定量聚合酶链式反应法检测各组移植瘤组织p53基因表达,2-ΔΔC t法计算各组p53基因的相对定量。结果各组移植瘤中p53基因相对表达的2-ΔΔC t值比较,差异无统计学意义(F=0.56,P=0.7557)。其中,CZBG中剂量联合ADM大剂量组p53基因相对表达量较高,而CZBG中剂量联合ADM小剂量组p53基因相对表达量较低。结论 CZBG与ADM联合使用能够上调P388移植瘤中p53抑癌基因的表达。Objective To study the impact of compound zhe - bei granule and adriamycin on p53 gene expression in the P388 xenograft tumor. Methods The tumor xenografis model was established by injecting the mouse lymphocytic leukemia cells （P388） in the subcurls of anterior axillary of Kunming mice, and then was treated with CZBG by intragastric administration and different doses of adriamyein by intraperitoneal injection （i. p. ）. After the end of the experiment, tumor was striped completely, and the expression of p53 gene in xenograft tumor of each group was detected by fluorescence quantitative polymerase chain reaction, and the relative quantification of p53 gene of each group was computed using 2 - -△△Ct method. Results Comparing the 2 - -△△Ct values of p53 gene relative expression of each group, no statistical significance was found （ F = 0.56, P = 0. 7557 ）. And the relative expression value of p53 gene of CZBG joint high - dose ADM group was higher, while the relative expression value of CZBG joint low - dose ADM group was lower. Conclusion Combined use of CZBG and ADM is able to raise the expression of p53 tumor suppressor gene in the P388 xenograft tumor.

关 键 词：复方浙贝颗粒 荧光定量聚合酶链式反应 P388细胞 移植瘤 p53 

分 类 号：R735.2[医药卫生—肿瘤]