沼泽型水牛Y染微卫星标记的筛选与多态性检测  

Screening and polymorphism of Y chromosome microsatellite markers in swamp buffalo

作　　者：张晓明[1] 乐祥鹏[1] 张春梅[1] 蓝贤勇[1] 陈宏[1] 雷初朝[1] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《遗传》2010年第3期242-247,共6页Hereditas（Beijing)

基　　金：教育部新世纪优秀人才支持计划项目(编号:NCET-07-0699)资助

摘　　要：为了研究水牛Y染色体的遗传多样性,文章以滇东南水牛3个地方群体——红河(HH)、西双版纳(BN)和普洱(PR)共31头公牛为研究对象,选取14个家牛Y染色体特异性微卫星标记,以检测这些标记在水牛Y染色体遗传多样性研究中的可行性。结果表明,3个标记(INRA008、UMN0103和UMN0504)只有1个等位基因,表现为单态;3个标记(UMN1113、UMN0304和BC1.2)均为3个等位基因,但呈单态;3个标记(UMN0920、UMN0307和UMN3008)呈现无规律的梯状条带,所以这9个标记都不适用于水牛的Y染色体遗传多样性研究;只有5个标记(INRA124、INRA189、BM861、PBR1F1和UMN2001)具有多态性,表明适用于水牛的Y染色体遗传多样性研究。这5个多态性Y染色体特异微卫星标记在滇东南水牛群体中的平均等位基因数(NA)为2.8000,平均期望杂合度(He)为0.3998,基因多样性(GD)为0.4144,多态信息含量(PIC)为0.3245,Shannon信息熵(SI)为0.5849,表明滇东南水牛群体的Y染色体具有中等遗传多态性。To understand the genetic diversity of buffalo Y chromosome, 14 microsatellite markers specific for cattle Y chromosomes were used to analyze 31 male buffaloes of three populations （i.e., HH, BN, and PR） originating from southeastern Yunnan Province, P. R. China. The aim of this study was to examine the practicability of cattle Y chromosome-specific microsatellite markers in studying genetic diversity of buffalo Y chromosome. Three markers （INRA008, UMN0103, and UMN0504） produced single allele, and three （UMN1113, UMN0304, and BC1.2） produced three alleles. But these markers were all monomorphic. Another three markers （UMN0920, UMN0307, and UMN3008） amplified irregular ladder-like bands. These markers were not suitable for investigating the swamp buffalo Y chromosome genetic diversity. The remaining five microsatellites （1NRA124, 1NRA189, BM861, PBR1F1, and UMN2001） were polymorphic, which can be used to study the swamp buffalo Y chromosome genetic diversity. The mean number of alleles （Na）, expected heterozygosity （He）, genetic diversity （GD）, polymorphism information content （PIC）, and Shannon＇s information index （SI） across these five polymorphic loci in the buffalo population samples studied were 2.8000, 0.3998, 0.4144, 0.3245, and 0.5849, respectively, demonstrating a moderate degree of polymorphism in the Y chromosome of the buffalo population.

关 键 词：水牛 Y染色体微卫星标记 遗传多样性 

分 类 号：S823[农业科学—畜牧学]