甲硫氨酸限制对胃癌细胞蛋白表达谱的影响  

Regulatory Effect of Methionine Restriction on Protein Expression Maps of Gastric Carcinoma Cells

作　　者：辛林[1] 曹伟新[2] 陈雪华[2] 刘炳亚[2] 朱正纲[2] 

机构地区：[1]南昌大学第二附属医院胃肠外科,330006 [2]上海交通大学附属瑞金医院消化外科研究所

出　　处：《肿瘤防治研究》2010年第3期278-280,共3页Cancer Research on Prevention and Treatment

基　　金：国家重点基础研究规划项目(973计划)(2002CB713703)

摘　　要：目的分析甲硫氨酸限制对胃癌SGC7901细胞蛋白表达谱的影响。方法Hoechst荧光染色法及流式细胞术验证胃癌SGC7901细胞的凋亡,用双向凝胶电泳分析蛋白表达差异。结果胃癌SGC7901细胞在限制甲硫氨酸培养液中培养5天后,部分细胞的细胞核内出现凋亡小体。限制甲硫氨酸培养液中SGC7901细胞的凋亡率显著高于正常培养液组(P<0.01)。双向电泳技术分析筛选出16个表达差异的蛋白点。结论甲硫氨酸限制可以导致胃癌SGC7901细胞部分蛋白表达增加或降低,进而诱导胃癌细胞凋亡。Objective To investigate the effects of methionine restriction treatment on the protein expression in human gastric carcinoma cell lines SGC-7901. Methods Cell apoptosis was validated by Hoechst fluorescent staining and flow cytometry test. The differences of protein expressions in SGC-7901 cells treated with methionine restriction were analyzed through the results from two-dimensional electrophoresis. Results Some apoptotic bodies were found in some gastric cancer cell lines cultured with M^- H^＋ medium,and the proportion of apoptotic cells in M H＋ medium was significantly higher than that in M^＋ H^- medium（P〈0. 01）. By two-dimensional electrophpresis, 16 protein spots were detected to be different. Conclusion The change of proteins expression in cells was directly reflected by two dimensional electrophoresis. The expressions of some proteins are increased and some reduced in SGC-7901 ceils after methionine restriction treatment.

关 键 词：甲硫氨酸限制 胃癌细胞 双向凝胶电泳 

分 类 号：R735.2[医药卫生—肿瘤]