共表达基因疫苗MAGE-1/IL-18的抗肿瘤免疫治疗作用  

Anti-tumor Immunotherapy Effect of Co-expression Genetic Vaccine MAGE-1/IL-18

作　　者：杨广民[1,2] 李首庆[1] 马寅芙[1] 魏兵兵[1] 时阳[2] 谭岩[2] 

机构地区：[1]吉林省人民医院中心实验室,长春130021 [2]吉林大学第一医院

出　　处：《肿瘤防治研究》2010年第3期294-297,共4页Cancer Research on Prevention and Treatment

基　　金：吉林省科技发展计划资助项目(200705449;200905192);吉林省卫生厅重点实验室资助项目

摘　　要：目的观察已构建的共表达基因疫苗pcIL-18-MAGE诱导特异性免疫应答的能力和抗肿瘤免疫治疗作用。方法共表达疫苗肌注小鼠,间隔十天加强免疫一次,共免疫三次,以pcMAGE-1、pcIL-18、pcDNA3和PBS为对照,检测小鼠脾细胞上清液IL-12和IFN-γ的浓度、脾细胞CTL杀伤活性及小鼠T细胞亚群情况;观察基因疫苗免疫MAGE(+)小鼠的成瘤时间和生存时间。结果pcIL-18-MAGE质粒免疫的小鼠,其脾细胞对SMMC-7721的杀伤率最高,与各对照组相比,差异有统计学意义;脾细胞CD3+、CD4+、CD8+T细胞和上清液中细胞因子IL-12和IFN-γ均明显升高。共表达疫苗免疫MAGE(+)小鼠后,小鼠成瘤时间明显延迟,生存时间明显延长。结论共表达基因疫苗pcIL-18-MAGE在实验动物体内有显著的诱导特异性抗肿瘤免疫作用,且对肿瘤的生成有一定的抑制作用。Objective To observe specific immune response and anti-tumor immunotherapy effect of the co-expression genetic vaccine MAGE/IL-18. Methods Co-expression genetic vaccine was injected to mice,two booster injections were carried out at ten days intervals. Using pcMAGE-1, pcIL-I8, pcDNA3 and PBS as comparison, IL-12, IFN-γ,CTL killing activity and T cell subgroup were investigated. Tumor growth and survival rates of vaccine immunized MAGE（ ＋ ） mice were recorded. Results The mice which were immunized by pcIL-18-MAGE, compared with control groups, had the highest CTL killing activity, CD3^＋ ,CD4^＋ ,CD8^＋ T cells,IL-12 and IFN-γ all step up obviously. The co-expression vaccine can delay tumor growth time and prolong the live time for mice. Conclusion Co-expression genetic vaccine pcIL-18- MAGE can induce conspicuous and has specific anti tumor immunotherapy effect on laboratory animals, and can significantly reduce the tumor growth.

关 键 词：基因疫苗 MAGE-1 IL-18 免疫应答 

分 类 号：R735.7[医药卫生—肿瘤]