P16表达缺失与胃肠道间质瘤生物学行为及预后关系  

The Relationship of Loss of P16 Expression with Biological Behaviors and Prognosis of Gastrointestinal Stromal Tumors

作　　者：赵占考[1] 姜忠敏[1] 何滔[1] 贾文娟[1] 孙保存[2] 

机构地区：[1]天津武警医学院附属医院病理科,天津市300162 [2]天津医科大学病理教研室,肿瘤医院病理科

出　　处：《中国肿瘤临床》2010年第5期257-259,共3页Chinese Journal of Clinical Oncology

基　　金：天津市科技发展计划项目资助(编号:043115211-1)~~

摘　　要：目的:探讨P16 mRNA和蛋白表达缺失与胃肠道间质瘤生物学行为及预后的关系。方法:采用反转录-聚合酶链式反应(RT-PCR)、免疫组织化学及Western Blot法分别检测胃肠道间质瘤组织中P16 mRNA和蛋白表达情况,并对患者进行随访,分析P16表达水平与胃肠道间质瘤细胞核增殖指数、肿瘤细胞的侵袭危险性及胃肠道间质瘤患者生存预后间的关系,采用SPSS13.0统计软件包进行统计学分析。结果:胃肠道间质瘤中P16 mRNA和蛋白的阳性表达率分别为53.8%(21/39,冰冻组织)、51.3%(20/39,冰冻组织)和51.4%(37/72,石蜡组织),不同侵袭危险性间比较差异有统计学显著性(P<0.05),随着侵袭危险性的增高,其表达率呈下降趋势;且与核增殖指数Ki-67表达及患者生存期呈负相关(P<0.05)。结论:P16表达缺失与胃肠道间质瘤的浸润进展有关;P16 mRNA和蛋白的检测有助于判断胃肠道间质瘤的生物学行为和预后。Objective： To investigate the relationship of loss of P16 expression with biological behaviors and prognosis of gastrointestinal stromal tumors （GISTs）. Methods： The expression of P16 protein and mRNA was detected in GISTs tissues by immunohistochemistry, Western blot and RT-PCR. The prognosis was evaluated through follow up. Results： The expression rates of P16 protein and mRNA in GISTs were 53.8% （21/39, Frozen tissue）, 51.3% （20/39, Frozen tissues） and 51.4% （37/72, Paraffin-embedded tissues）, respectively. The expression of P16 was significantly different among GISTs of different aggressive risk （P〈0.05）. With the incease of aggressive risk, the expression of P16 was deceased. The expression of P16 was negtively correlated with Ki-67 and patient survival （P〈0.05）. Conclusion： The loss of P16 expression has a positive correlation with the infiltration and progression of GIST. Detection of P16 protein and mRNA is helpful for the evalutaion of biological behaviors and prognosis of gastrointestinal stromal tumors.

关 键 词：胃肠道间质瘤 P16 免疫组织化学 反转录-聚合酶链式反应 

分 类 号：R735[医药卫生—肿瘤]