凋亡调节蛋白Bcl-2、p53和Caspase-3在乳腺癌组织中的表达及其相互关系  

The relationship of expressions of bcl-2,p53 and caspase-3 in human breast cancer

作　　者：李艳萍[1] 王琳[2] 伍思琪[1] 吴爽[1] 

机构地区：[1]广东药学院组胚教研室,广东广州510006 [2]新乡医学院组织学与胚胎学教研室,河南新乡453003

出　　处：《解剖科学进展》2010年第2期167-170,共4页Progress of Anatomical Sciences

基　　金：河南省教育厅科技攻关课题(20023100008)

摘　　要：目的通过观察人乳腺癌组织中凋亡调节蛋白p53、bcl-2和caspase-3表达,探讨bcl-2、p53和caspase-3在乳腺癌发生、发展过程中的作用及其相互关系,为乳腺癌的生物治疗提供实验依据。方法收集手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法和图像分析技术对21例乳腺癌标本进行检测。结果乳腺癌组织中bcl-2蛋白阳性表达率(52.4%,11/21)明显高于相对正常乳腺组织(19.0%,4/21);乳腺癌组织中p53蛋白阳性表达率(57.1%,12/21)也明显高于相对正常乳腺组织(0%);而乳腺癌组织中caspase-3蛋白阳性表达率(38.0%,8/21)明显低于相对正常乳腺组织(76.1%,16/21)。结论p53、bcl-2和caspase-3蛋白在乳腺癌发生、发展过程中发挥着既独立又协同的作用。Objective To investigate the relationship of the expressions of bcl-2,p53 and caspase-3 in human breast cancer.Methods The human breast cancer tissues and relatively normal breast tissues were collected from surgical operation and the samples of 21 patients with breast cancer were analyzed by immunocytochemistry and computer imaging technique.Results The expression level of bcl-2 protein in human breast cancer（52.4%,11/21）was significantly higher than that in normal breast tissues（19.0%,4/21）.The expression level of p53 protein in breast cancer（57.1%,12/21） was also significantly higher than that in normal breast tissues（0%,0/21）.However,the expression level of caspase-3 protein in human breast cancer（38.0%,8/21）was significantly lower than that in normal breast tissues（76.0%,16/21）.Conclusion Bcl-2,p53 and caspase-3 may play an independent or synergetic roles in the development of breast cancer.

关 键 词：BCL-2 P53 CASPASE-3 免疫组织化学 乳腺癌 人 

分 类 号：R737.9[医药卫生—肿瘤]