热凝脑膜中动脉对硝酸甘油致偏头痛大鼠三叉神经尾核c-fos基因表达的影响  

Effects of heating coagulation of the middle meningeal artery on c-fos expression in the trigeminal caudalis nucleus in migraine rats triggered by nitroglycerin

作　　者：朱晓凤[1] 韩月臣[1] 熊文萍[1] 逯素梅 李建峰 王海波[1,2] 樊兆民[1] 

机构地区：[1]山东大学附属省立医院耳鼻咽喉-头颈外科,济南250021 [2]山东省临床医学研究院眼耳鼻喉科学研究所,济南250021

出　　处：《山东大学耳鼻喉眼学报》2010年第1期61-64,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：山东省科技攻关计划课题(2007GG20002)

摘　　要：目的探讨热凝脑膜中动脉对硝酸甘油诱发的偏头痛大鼠三叉神经尾核c-fos基因表达的影响。方法48只健康雌性Wistar大鼠随机分为4组(每组12只):生理盐水组(A组),硝酸甘油组(B组,10mg/kg),假手术组(C组),手术组(D组)。观察各组大鼠行为学表现,成功建模后每组随即平分为2小组分别采用RT-PCR和West-ernblot法检测大鼠三叉神经尾核c-fos基因表达。结果各组大鼠挠头、爬笼及耳红等行为学评分(x珋±s)分别为2.33±0.74,39.57±5.44,37.87±4.68,15.6±3.30。4组大鼠三叉神经尾核c-fosmRNA相对表达水平分别为0.50±0.08,0.94±0.12,0.91±0.09,0.71±0.08;c-fos蛋白相对表达水平分别为0.57±0.08,0.93±0.09,0.93±0.09,0.73±0.11。A组大鼠三叉神经尾核c-fosmRNA和蛋白相对表达水平低于B组,C组和D组,且D组大鼠三叉神经尾核c-fosmRNA和蛋白相对表达水平低于B组和C组,差异有统计学意义(P<0.05),但B组与C组之间差异无统计学意义(P>0.05)。结论热凝脑膜中动脉可显著减轻硝酸甘油诱发的大鼠偏头痛症状,表明脑膜中动脉在硝酸甘油诱导的大鼠偏头痛模型致痛过程中起主要作用,这可能是其通过降低硝酸甘油诱导的大鼠三叉神经尾核c-fos基因表达,减少或抑制三叉神经尾核神经元的激活引起的。Objective To detect the influence of heating coagulation of the middle meningeal artery on c-fos expression in the trigeminal caudalis nucleus in migraine rats triggered by nitroglycerin. Methods Forty-eight female Wistar rats were randomly assigned to four groups, the isotonic saline group （ group A）, the nitroglycerin group （ group B）, the operation group （group C）, and the sham operation group （group D）, with 12 subjects in each group and each group was subdivided into another two equal groups after successfully constructing the animal model. C-fos expression in the trigeminal caudalis nucleus was detected by means of RT-PCR and Western blot respectively. Results Injection of 10 mg/kg nitroglycerin was able to trigger the animal model of migraine as evidenced by the appearances of a series of symptoms, such as frequently head scratching, climbing the cage and red ear. The scores of these behaviors for each group were respectively 2.33 ± 0.74, 39.57 ± 5.44, 37.87 ± 4.68, 15.6 ± 3.30. At the same time, c-fos expressions in the trigeminal caudalis nucleu in group B, group C, or group D was significantly increased compared with that of group A （ P 〈 0.05 ）. More importantly, c-fos expression in the trigeminal caudalis nucleus in group D was much lower than that in group B and group C （ P 〈 0.05 ）, but there was no statistical difference between group B and group C （ P 〉 0.05）. Conelusions Results show that heat coagulation of the middle meningeal artery may attenuate head complaint of rats induced by nitroglycerin, and it is concluded that the middle meningeal artery plays a primary role in the pathophysiology of the rat migraine model triggered by nitroglycerin and the efficacy may play a part in decreasing c-fos expression and containing activation of neurons in the trigeminal caudalis nucleus.

关 键 词：偏头痛 脑膜中动脉 三叉神经尾核 c—fos基因 大鼠 WISTAR 

分 类 号：R747.2[医药卫生—神经病学与精神病学]