宫内感染致脑瘫大鼠运动功能不同评定方法的比较  

Comparison of Two Kinds of Evaluation for Motor Function of Cerebral Palsy Neonatal Rats Caused by Intrauterine Infection

作　　者：郭岚敏[1] 李晓捷[1] 庞伟[1] 

机构地区：[1]佳木斯大学附属第三医院,佳木斯大学儿童神经康复实验室,黑龙江佳木斯市154003

出　　处：《中国康复理论与实践》2010年第3期224-227,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：黑龙江省研究生创新科研资金项目(YJSCX2008-061HLJ);佳木斯大学科学技术研究项目(S2009-041)

摘　　要：目的研究宫内感染致脑瘫大鼠运动功能评价的最佳方法。方法受孕17d的48只孕鼠脂多糖(LPS)450μg/kg腹腔注射(LPS组),对照组孕鼠腹腔注射同等剂量的生理盐水。随机选取对照组活产足月仔鼠60只(A组),LPS组活产足月仔鼠120只,将LPS组仔鼠随机分为干预组(B1)60只和非干预组(B2)60只。干预组进行早期干预,非干预组和对照组常规饲养。25日龄各组进行神经行为学检测,鉴定脑瘫鼠,B1组中检测出的脑瘫鼠(B1CP组)继续早期干预,B2组中检测出的脑瘫鼠(B2CP组)常规饲养,A组中随机选取10只鼠作为对照组(A′组)常规饲养,其余鼠常规处死;25日龄、42日龄时,A′组、B1CP组和B2CP组均进行神经行为学检测及运动功能评定。结果25日龄仔鼠神经行为学检测B1组中鉴定出7只脑瘫鼠,B2组中鉴定出13只脑瘫鼠,A组中无脑瘫鼠。B1CP组25日龄与42日龄各项检测结果比较,悬吊试验、斜坡试验、旷场实验、拒俘反应有非常显著性差异(P<0.01);改良的BBB运动功能评分有非常显著性差异(P<0.01);B2CP组和A′组25日龄与42日龄各项检测结果比较无显著性差异。结论联合应用悬吊试验与改良BBB运动功能评定法可用于宫内感染致脑瘫鼠运动功能的评定。Objective To study the optimum method to evaluate the motor function of cerebral palsy neonatal rats caused by intrauterine infection. Methods 48 Wistar 17 d pregnant rats were consecutively injected with lipopolysacchide （LPS） （450 μg/kg） for 2 d （LPS group）, and other 10 Wistar 17 d pregnant rats （control group） were injected with the same dose of saline. The neonatal rats were selected randomly in control group （A） （n= 60） and LPS group （n= 120）, the latter was divided into intervention group （B1, n=60） and nonintervention group （B2,n= 60）. The CP rats were identified with neurobehavior detection on the 25th day. Then the CP rats in the B1 group （B1CP） continued their intervention, the CP rats in the B2 group （B2CP） and 10 rats random from group A （A＇） were raised routinely. They were assessed with neurobehavior detection and improved BBB assessment on the 25th and 42nd day. Results There were 7 CP rats in B1 group, and 13 in B2 group. There was significant difference in the scores of hanging test, slopes test, open-field experiment, resist captured reaction between the 25th and 42nd day in B1CP group （P〈0. 01）, as well as in improved BBB assessment （P〈0. 61）, but not in neurobehavior detection; while there was not significant difference in B2CP group and in A＇ group in all the assessment above. Conclusion Not neurobehavior detection, but hanging test and BBB assessment, can be used to evaluate the motor function of cerebral palsy rats caused by intrauterine infection.

关 键 词：脑性瘫痪 宫内感染 早期干预 运动功能 BBB评分 

分 类 号：R742.3[医药卫生—神经病学与精神病学]