聚乙二醇-牛血红蛋白在体外循环中对炎性因子的影响  

Effect of Polyethylene Glycol Conjugated Bovine Hemoglobin (PEG-bHb) on inflammatory factors during Cardiopulmonary Bypass in sheep

作　　者：高国栋[1] 龙村[1] 刘晋萍[1] 贺学英[2] 张晓伟[2] 管博[2] 王蕊 唐越[3] 刘谦[2] 

机构地区：[1]中国医学科学院阜外心血管病医院体外循环科,北京100037 [2]"863计划"人血代用品产业化基地,北京100085 [3]中国医学科学院阜外心血管病医院实验外科,北京100037

出　　处：《中国分子心脏病学杂志》2010年第1期33-35,共3页Molecular Cardiology of China

摘　　要：目的采用颈静脉-股动脉部分转流的体外循环(CPB)模式,观察以聚乙二醇-牛血红蛋白(PEG-bHb)代替预充液中的胶体溶液预充进行的体外循环中炎性因子的变化。方法选取13只小尾寒羊,根据CPB预充液成分的不同随机分为两组:实验组(P组,n=7)在体外循环前经颈静脉放自体血(15%)后,在体外循环晶体预充液中加入等量PEG-bHb,在CPB转流结束后,将保存的羊自体血输入体内;对照组(C组,n=6)应用常规体外循环预充(706羟乙基淀粉和乳酸林格氏液)方案。分别在CPB前(T1)、CPB开始30min(T2)、CPB结束后1h(T3)、4h(T4)、8h(T5)、24(T6)、48h(T7)各时点抽取动脉血样本。ELISA法检测血清白介素6(IL-6)、白介素8(IL-8)和肿瘤坏死因子(TNF-浕)浓度。结果组内与术前相比,所有细胞因子均呈现上升趋势;组间比较,两组各时点炎性因子浓度无显著性差异。结论PEG-bHb作为一种有效的血液替代品,不会增加体外循环炎性因子的产生,可安全应用于体外循环预充。Objective To evaluate the Effect of Polyethylene Glycol Conjugated Bovine Hemoglobin （PEG-bHb） primed into the Cardiopulmonary Bypass（CPB）circuits compared to crystalloid/collid prime solution on the release of inflammatory factor during Cardiopulmonary Bypass in sheep.Methods the partial CPB by jugular vein-femoral artery model was used to evaluate PEG-bHb effects on inflammatory factor as colloid substitute in prime solution.Thirteen sheep were divided randomly into two groups.In group P（n=7）,9ml/kg PEG-bHb was added in the crystalloid prime,and equal volume of autologous animal whole blood was harvested before CPB from jugular vein,and then the harvested blood was transfused into the same animal after CPB.In group C（n=6）,which was served as control group,a crystalloid/collid solution was used as prime solution.Blood samples were taken before CPB（T1）,30 min after CPB begin（T2）,1h（T3）、4h（T4）、8h（T5）、24（T6）、48h（T7）after CPB ended.The concentration of tumor necrosis factor-a（TNF-a）,interleukin-6（IL-6） and interleukin-8（IL-8） in plasma level were measured.ResultsThe concentration of TNF-a,IL-6 and IL-8 levels in plasma of two groups showed no difference at each point.But they all increased after CPB in each gpoup.Conclution PEG-bHb don＇t increase the levels of inflammatory factors in plasma and can be safe used during cardiopulmonary bypass as a component in the prime solution.

关 键 词：体外循环 炎性因子 PEG-bHb 牛血红蛋白 聚乙二醇 预充液 羟乙基淀粉 细胞因子 肿瘤坏死因子 血液替代品 

分 类 号：R363[医药卫生—病理学]