医用几丁糖对角膜碱烧伤后新生血管的抑制作用  

Inhibitory effects of chitosan on corneal neovascularization after corneal alkali burn

作　　者：白瑜[1] 周忠友[1] 刘晓坤[1] 赵平[1] 

机构地区：[1]河北医科大学第三医院眼科,河北石家庄050051

出　　处：《眼外伤职业眼病杂志》2010年第3期166-169,共4页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的观察医用几丁糖对角膜碱烧伤后新生血管的抑制作用。方法NaOH烧伤兔角膜制作角膜碱烧伤模型,随机分为碱烧伤组(碱烧伤)和几丁糖组(碱烧伤+2%医用几丁糖滴眼)。分别于1、4、7、14d观察两组兔角膜新生血管生长情况,HE染色计算多形核粒细胞(PMN)数量,兔疫组化染色测定血管内皮生长因子(VEGF)的活性。结果几丁糖组新生血管较碱烧伤组生长缓慢,PMN计数及VEGF表达较碱烧伤组均明显减少。结论医用几丁糖可抑制碱烧伤后角膜新生血管的形成。Objective To observe the inhibitory effect of Chitosan on corneal neovascularization after corneal alkaliburn. Methods Corneal alkali-burn model of rabbits was made by burning right eyes with NaOH and divided into two groups in random, Cautery group（cautery） and Chitosan group （cautery and put drops in the eyes with 2% chitosan）. CNV of the two groups were observed, PMNs were counted and the contents of VEGF were measured at the 1st, 4th, 7th, 14th day. Results Compared with the Cantery groups the growth of CNV was slowly, the counts of PMN and the expressions of VEGF were both decreased significantly. Conclusion The Chitosan can inhibits the CNV after corneal alkali bum.

关 键 词：几丁糖 角膜新生血管 多形核粒细胞 血管内皮生长因子 

分 类 号：R779.1[医药卫生—眼科]