荷瘤大鼠氩氦冷冻治疗前后免疫功能的变化  

Alteration of immune function of rats with implanted breast cancer after Ar-He cryoblation

作　　者：刘建刚[1] 葛成林 谭莹 张风庆 李淑芳 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心 [2]山东省潍坊市肿瘤医院介入治疗中心

出　　处：《重庆医学》2010年第7期784-786,I0001,共4页Chongqing medicine

摘　　要：目的通过检测荷瘤SD大鼠经冷冻消融治疗前后T细胞亚群(CD3+、CD4+、CD8+)、CD4+/CD8+T细胞比值及其外周血sIL-2R含量的变化情况,研究荷瘤大鼠治疗前后机体免疫功能的变化。方法建立SD大鼠W-256肿瘤细胞皮下移植瘤模型,将40只试验大鼠分为4组:正常对照组、荷瘤对照组、冷冻治疗组、手术治疗组,每组10只。应用流式细胞仪检测其治疗前,治疗后l、3、5周的T细胞亚群(CD3+、CD4+、CD8+)、CD4+/CD8+T细胞比值变化,应用免疫试剂盒检测外周血sIL-2R含量变化。结果荷瘤大鼠治疗前(CD3+、CD4+)、CD4+/CD8+T细胞比值均低于正常对照组,而CD8+T细胞数及外周血sIL-2R的表达水平均高于正常对照组,差异有统计学意义(P<0.05)。经冷冻消融治疗后冷冻治疗组大鼠的(CD3+、CD4+)、CD4+/CD8+T细胞比值均有明显回升,在术后各个时间点均与同时期荷瘤对照组比较差异有统计学意义(P<0.05)。而CD8+T细胞和外周血sIL-2R含量则明显下降,在术后各个时间点均与同时期荷瘤对照组比较差异有统计学意义(P<0.05)。冷冻治疗组在治疗后第1、3周(CD3+、CD4+)、CD4+/CD8+T细胞比值均高于同期手术治疗组,而CD8+T细胞数及外周血sIL-2R的表达水平均低于同期手术治疗组,差异有统计学意义(P<0.05)。结论荷瘤大鼠免疫功能低下,经冷冻消融治疗后可在短期内显著提高大鼠免疫功能,具有免疫激活作用,使机体的免疫功能得到一定程度的改善。Objective To study the change of immune function in rats with implanted breast cancer after Ar-Hc cryoblation. Methods A total of 40 SD rats were randomized into 4 groups：cryoblation, surgical resection, tumor bearing control group,and normal control group. The contents of T cell Phenotype in blood were detected with flow cytometry（FCM） and the contents of sIL- 2R were measured by ELISA before and after cryoblation. Results Before cryoblation, CD3^＋ ,CD4^＋ , CD8^＋ T celI,CD4^＋/CD8^＋ and sIL- 2R in the group of tumor-bearing control rats were significantly different than those in the normal control group. After cryoblation,CD3^＋ ,CD4^＋ ,CD4^＋/CD8^＋ in the cryoblation rats model increased significantly（P〈0.05）compared with the group of tumor-bearing control, and it showed significantly different （P 〈 0.05） and CD8^＋ T cell. expression of sIL-2R decreased. It also showed significantly different compaved with the group of surgical resection after 1,3 wecks（P〈0.05）. Conclusion Cryoblation can efficiently stimulate splenocyte activation and Proliferation in tumor-bearing mice,and enhance splenocyte cytotoxicity to tumor cells, cryoblation may activate and improve cell-mediate immune function to some extent.

关 键 词：氩氦冷冻消融 种植性乳腺癌 免疫功能 

分 类 号：R73-3[医药卫生—肿瘤]