Preparation of new tissue engineering bone-CPC/PLGA composite and its application in animal bone defects  

作　　者：郑治 王剑龙 米雷 刘咏 侯光辉 陈旦 黄伯云 

机构地区：[1]The Third Xiangya Hospital,Central South University [2]State Key Laboratory of Powder Metallurgy,Central South University [3]Xiangya School of Medicine,Central South University

出　　处：《Journal of Central South University》2010年第2期202-210,共9页中南大学学报（英文版）

基　　金：Projects(30370412, 30670558) supported by the National Natural Science Foundation of China

摘　　要：To investigate the feasibility of implanting the biocomposite of calcium phosphate cement(CPC)/polylactic acid-polyglycolic acid(PLGA) into animals for bone defects repairing,the biocomposite of CPC/PLGA was prepared and its setting time,compressive strength,elastic modulus,pH values,phase composition of the samples,degradability and biocompatibility in vitro were tested.The above-mentioned composite implanted with bone marrow stromal cells was used to repair defects of the radius in rabbits.Osteogenesis was histomorphologically observed by using an electron-microscope.The results show that compared with the CPC,the physical and chemical properties of CPC/PLGA composite have some differences in which CPC/PLGA composite has better biological properties.The CPC/PLGA composite combined with seed cells is superior to the control in terms of the amount of new bones formed after CPC/PLGA composite is implanted into the rabbits,as well as the speed of repairing bone defects.The results suggest that the constructed CPC/PLGA composite basically meets the requirements of tissue engineering scaffold materials and that the CPC/PLGA composite implanted with bone marrow stromal cells may be a new artificial bone material for repairing bone defects because it can promote the growth of bone tissues.To investigate the feasibility of implanting the biocomposite of calcium phosphate cement （CPC）/polylactic acid-polyglycolic acid （PLGA） into animals for bone defects repairing, the biocomposite of CPC/PLGA was prepared and its setting time, compressive strength, elastic modulus, pH values, phase composition of the samples, degradability and biocompatibility in vitro were tested. The above-mentioned composite implanted with bone marrow stromal cells was used to repair defects of the radius in rabbits. Osteogenesis was histomorphologically observed by using an electron-microscope. The results show that compared with the CPC, the physical and chemical properties of CPC/PLGA composite have some differences in which CPC/PLGA composite has better biological properties. The CPC/PLGA composite combined with seed cells is superior to the control in terms of the amount of new bones formed after CPC/PLGA composite is implanted into the rabbits, as well as the speed of repairing bone defects. The results suggest that the constructed CPC/PLGA composite basically meets the requirements of tissue engineering scaffold materials and that the CPC/PLGA composite implanted with bone marrow stromal cells may be a new artificial bone material for repairing bone defects because it can promote the growth of bone tissues.

关 键 词：BIOMATERIALS mesenchymal stem cells （MSCs） calcium phosphate cement （CPC） polylactic acid-polyglycolic acid （PLGA） bone defects 

分 类 号：R318.0[医药卫生—生物医学工程]