蛋白质粉对小鼠免疫功能的影响  

The Effects of Protein Powder on Immunity Function of Mice

作　　者：李立[1] 王宁[2,3] 张桂霞[1] 张焱[1] 王海玉[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450016 [2]国家人口计生委科学技术研究所,北京100081 [3]协和医科大学,北京100730

出　　处：《中国比较医学杂志》2010年第3期54-56,60,共4页Chinese Journal of Comparative Medicine

摘　　要：目的探讨蛋白质粉对正常小鼠免疫调节作用。方法将BALB/c小鼠随机分为3批,每批分为4组,分别进行了小鼠免疫器官/体质量比值测定和小鼠碳廓清实验;绵羊红细胞诱导小鼠DTH、抗体生成细胞检测和血清凝血素测定(HC50);ConA诱导的小鼠脾淋巴细胞转化实验和乳酸锂脱氢酶法(LDH)测定NK细胞活性;小鼠腹腔巨噬细胞吞噬鸡红细胞实验。结果10.00 g/kg剂量的蛋白质粉可增强绵羊红细胞诱导小鼠DTH能力(P<0.05),促进抗体生成细胞数的生成(P<0.01)。3.33 g/kg和10.00 g/kg剂量组能促进ConA诱导的小鼠脾淋巴细胞转化能力(P<0.05或P<0.01)和血清凝血素的生成(P<0.05);三个剂量组均能提高小鼠腹腔巨噬细胞吞噬鸡红细胞能力(P<0.05或P<0.01);3.33 g/kg和10.00 g/kg剂量组能提高NK细胞活性(P<0.05);但对小鼠碳廓清能力和免疫器官/体重比值无明显影响。结论蛋白质粉对正常小鼠的细胞、体液免疫和单核-巨噬细胞功能和NK功能有促进作用,即具有增强免疫力功能。Objective To explore the effect of protein powder on immunological function.Methods／ The BALB/c mice were divided into three groups,and each group was divided into four groups.And individually to test the ratio of spleen-thymus/body weight,T lymphocyte proliferation of mouse spleen induced by ConA,the response of delayed hypersenitivity,the amount of hemolytic antibility in serum, antiboty-producing cell assay,phagocytic activity of macrophages and the ability of macrophages for carbon particle clearance and cytotoxicity of NK cells by LDH method.Results Compared with the control group,the 10.00 g/kg dose group could increase the response of delayed hypersenitivity induced by SRBC（P〈0.05） and the quantity of antibody-producing cells（P〈0.01）.The dose groups of 3.33 g/kg and 10.00 g/kg could promote the ability of T lymphocyte proliferation of mouse spleen induced by ConA and expurgation effect（P〈0.05 or P〈0.01） and the amount of hemolytic antibility in serum（P〈0.05）;The three doses could enchance phagocytic activity of macrophages and the ability of macrophages for carbon particle clearance（P〈0.05 or P〈0.01）;The dose groups of 3.33 g/kg and 10.00 g/kg could stimulate strengthening the activity of natural killer cells（P〈0.05）.But significant difference was not observed in the ratio of spleen-thymus/body weight and the capacity of the carbon clearance of the spleep macrophages.Conclusions／ Protein powder can promote effects on cell immumity,humoral immumity,the macrophage engulfining function and NK cell cytotoxicity,so it has the function of enhancing immunity.

关 键 词：蛋白质粉 免疫调节 小鼠 

分 类 号：R33[医药卫生—人体生理学]