TSA对Tca-8113细胞抑制作用及端粒酶的影响  

The effects of TSA on the inhibition of Tca-8113 cell and on telomerase

作　　者：韩斯琴高娃[1] 毛立民[1] 张冰[1] 曲正[1] 李国松[1] 郭福林[1] 

机构地区：[1]哈尔滨医科大学附属第一医院口腔颌面外科,150001

出　　处：《现代口腔医学杂志》2010年第2期117-120,共4页Journal of Modern Stomatology

基　　金：黑龙江省教育厅科学技术研究项目(11511168);哈尔滨市科学技术局(2005AFXXJ050)

摘　　要：目的观察不同浓度的组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)对口腔鳞癌细胞系Tca-8113细胞体外抑制作用及端粒酶的影响,从而可能提供TSA对口腔鳞癌临床化疗应用的实验依据。方法采用不同浓度TSA对Tca-8113细胞进行药物干预,倒置显微镜观察细胞用药前后的形态学改变;MTT法检测细胞增殖活性,确定后续实验中TSA作用浓度及时间点;应用以PCR为基础的端粒重复序列扩增(TRAP-PCR)银染法检测端粒酶活性,用蛋白印迹(Western blot)方法检测端粒酶逆转催化亚基单位(hTERT)蛋白表达。结果TSA呈时间剂量性抑制Tca-8113细胞的生长,同时它也能降低端粒酶活性及hTERT蛋白表达的变化趋势。结论TSA能够抑制Tca-8113细胞的增殖,进而下调细胞端粒酶活性及hTERT蛋白表达,这可能是TSA抗肿瘤作用机制之一。Objective To investigate the inhibitive effects of trichostation A（TSA） with different concentrations on oral cancer Tca-8113 cells and telomerase activity,and to provide experimental proof for the clinical applications of TSA in anticancer chemotherapy.Methods Tca-8113 cells were treated by TSA with different concentrations,and morphology of cells was examined by transmission electron microscope;proliferative activity of cells was assessed by using MTT so that the concentration of TSA and the time-point could be determined for the follow-up experiment;Telomerase activity was examined by using TRAP-PCR,and hTERT protein was analyzed by Western blotting.Results A time-and dose-dependent inhibition was detected in Tca-8113 treated by TSA.Both the telomerase activity and the expression level of hTERT decreased after TSA treatment.Conclusion The results indicated that TSA can effectively inhibit the proliferation of Tca-8113 cells and thus reduce the telomerase activity and expression level of hTERT,which might be one of the mechanisms for the anticancer function of TSA.

关 键 词：曲古抑菌素A 口腔鳞癌细胞 端粒酶 

分 类 号：R739.8[医药卫生—肿瘤]