连续培养猪牙乳头细胞的免疫组化分析  

Immunohistochemistry analysis of porcine dental pappilla cells cultured in vitro

作　　者：傅远飞[1] 张富强[1] 吴伟[1] 徐侃[1] 翁雨来[1] 李静[1] 张秀丽[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔修复科.上海市口腔医学重点实验室,200011

出　　处：《现代口腔医学杂志》2010年第2期135-137,共3页Journal of Modern Stomatology

基　　金：上海市科学技术委员会项目(08DZ2271100);上海市重点学科(特色学科)建设项目(T0202);上海市重点学科建设项目(S30206);上海市卫生局项目(2008y101);上海交通大学"医工(理)交叉研究基金"(YG2008MS37)

摘　　要：目的探讨连续培养的牙乳头细胞的功能学特征。方法连续培养钟状期猪牙乳头细胞,应用间接法免疫组化检测各代细胞的牙本质涎蛋白(DSP)、核心结合因子1(Cbfa1)、Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、骨桥蛋白(OPN)、骨粘蛋白(ON)、骨钙蛋白(OCN)等的表达情况,并以第4代猪骨髓基质细胞及人成纤维细胞作为对照组。结果免疫组化结果可见连续培养的各代猪牙乳头细胞均表达DSP、ColⅠ及ON,所有各代猪牙乳头细胞均未见OCN表达,ColⅢ只在第1代的少量细胞中有表达,Cbfa1及OPN在各代细胞中均有表达。结论体外培养的猪牙乳头细胞具有旺盛的分泌能力,可能具有形成钙化组织的潜能和形成牙本质样结构的可能性。Objective To investigate the characteristics of protein expression of the porcine dental papilla cells（pDPCs） cultured in vitro.Methods pDPCs were continuous cultured by enzyme digestion method.Then immunochemistry method was performed to detect the expression of DSP,Cbfa1,Col Ⅰ,Col Ⅲ,OPN,ON,and OCN.Results The immunohistochemistry results show that DSP,ColⅠ and ON were postively expressed in every generation pDPCs.There was no OCN expression in the cells of every generation.As to ColⅢ,only weak positive expression was detected in the cells of1st generation.Cbfa1 and OPN were positively expression in every generation cells.Conclusion The pDPCs cultured in vitro have strong hearty ability of protein secretion and possibility of forming calcification tissues or dentin-like hard tissues.

关 键 词：牙乳头细胞 免疫组化 牙本质涎蛋白 

分 类 号：R780.2[医药卫生—口腔医学]