基于SPR生物传感器的抗生素残留检测及影响因素分析  

Analysis of Influencing Factors on Detection of Antibiotics Residues Based on Surface Plasmon Resonance Biosensor

作　　者：石婷[1] 刘瑾[1] 张婉洁[1] 苏洋[1] 张增福[1] 徐可欣[1] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072

出　　处：《天津大学学报》2010年第3期255-261,共7页Journal of Tianjin University(Science and Technology)

基　　金：国家自然科学基金资助项目(30700146;30800239);高等学校博士点专项科研基金资助项目(20070056076;20070056075);天津市应用基础与前沿技术研究计划资助项目(06YFGDGX17400)

摘　　要：为了检测抗生素提出了采用生物分子相互作用原理的检测方法.采用基于表面等离子体共振技术的光学传感器,通过自组装分子技术将单克隆抗体固定到传感器金膜表面用于检测对应抗原,即待测抗生素,并记录传感器表面液体折射率的变化曲线,其反映了被吸附抗生素的浓度的大小.对不同浓度的氨苄青霉素水溶液进行了测定,得到该传感器的工作曲线,整体工作曲线呈单调上升,且线性关系较好,同时得到了该检测方法的最低检测极限为1.25ng/mL,明显低于欧盟规定的最大残留限量(4μg/kg).进一步分析了流速、温度、pH值和离子浓度对检测的影响,获得了该检测方法的最佳测量条件,提高了该方法的检测精度,从而保障了该方法的有效性.A method based on bio-molecular interaction analysis （BIA） was proposed for the detection of antibi- otics residues in milk by adopting the surface plasmon resonance （SPR） biosensor. Monoclonal antibodies were immobilized on the gold surface of SPR biosensor to detect corresponding antigens-antibiotics. Refractive index （RI） curves of different liquids, which are recorded by SPR biosensor, are related to concentrations of antibiotics in milk. Water solution with a series of ampicillin concentrations was detected and analyzed in this paper. The whole working curve with good linearity of the sensor was acquired, The lowest limit using this method reached 1.25 ng/mL, which was much lower than maximum residue limit （MRL： 4μg/kg） set by European Union. Furthermore, the influencing factors such as flow velocity of liquid, temperature, pH range and ion concentrations were discussed in order to get the optimum experiment conditions for the method and ensure its effectiveness and high accuracy.

关 键 词：抗生素残留 表面等离子体共振 免疫测定 生物分子相互作用 

分 类 号：O657[理学—分析化学]