应用实时荧光定量PCR检测结直肠癌与癌旁组织中miRNA-221的差异表达状况  

Expression of miRNA-221 in colorectal carcinoma and non-tumorous tissues by using real-time quantitative PCR

作　　者：曾俊杰[1] 孙凯[1] 吴承堂[1] 雷尚通[1] 王伟[1] 

机构地区：[1]南方医科大学南方医院,广州510515

出　　处：《山东医药》2010年第12期26-28,共3页Shandong Medical Journal

基　　金：广东省自然科学基金博士启动项目(8451051501000390);南方医科大学南方医院院长基金(2008C005)

摘　　要：目的分析结直肠癌与毗邻癌旁组织中miRNA-221差异表达状况。方法常规抽提30例结直肠癌及对照癌旁组织中总RNA,应用实时荧光定量PCR方法检测标本中miRNA-221的表达状况。结果与癌旁组织相比,结直肠癌组织中miRNA-221表达明显上调(P<0.01)。结论实时荧光定量PCR是一种较精确的miRNA定量检测方法;miRNA-221可能通过转录后基因沉默机制促进结直肠癌的发生发展。Objective To investigate the different expression of miRNA-221 in colorectal carcinoma and adjacent ontumorous tissues. Methods Thirty colorcctal carcinoma and adjacent non-tumorous tissues were collected respectively and the total RNA was isolated routinely. The miRNA-221 expression was detected by using real-time quantitative polymerase chain reaction （PCR）. Results The expression of miRNA-221 was significantly increased in coloreetal carcinoma tissues than those in adjacent non-tumorous tissues （P 〈0.01 ）. Conclusion The real-time quantitative PCR assay is an accurate, rapid and less-expensive method to detect miRNA, miRNA-221 could promote colorectal carcinoma occurrence by means of post-transcriptional gene silence.

关 键 词：结直肠肿瘤 miRNA-221 实时荧光定量 聚合酶链反应 

分 类 号：R735.3[医药卫生—肿瘤]