肠易激综合征模型大鼠肺组织SP含量及基因表达变化研究  

SP Content of the Lung Tissue in Model Rats with Irritable Bowel Syndrome and Changes of Gene Expression

作　　者：张锋雷[1] 王艳杰[1] 柴纪严[1] 赵金如[1] 李任锋[1] 李丹[1] 刘旭东[1] 王德山[1] 宋京京[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]广西中医学院,广西南宁530001

出　　处：《河南中医》2010年第4期348-350,共3页Henan Traditional Chinese Medicine

基　　金：国家973课题(编号:2007CB512702)

摘　　要：目的:检测肠易激综合征(IBS)模型大鼠肺组织P物质(SP)表达变化,探讨中医肺与大肠相表里脏腑相关理论的科学内涵。方法:采用慢性应激刺激与束缚相结合的方法建立IBS模型大鼠,复制成功后分别以免疫组化、酶联免疫吸附试验(ELISA)和逆转录多聚酶链反应(RT-PCR)等方法检测肺组织中的P物质(SP)表达。结果:与对照组比较IBS模型组大鼠的肺组织中SP含量以及基因表达明显增高(P<0.01)。结论:IBS状态下大鼠肺组织中SP的含量及基因表达发生上调,提示肺与大肠通过神经-内分泌系统存在着内在联系。Objective ： To detect the expression changes Of lung tissue substance P （SP） of model rats with irritable bowel syndrome （IBS）, to explore the scientific connotation of the relative theory of the lung having exterior and interior relationship with large intestine. Methods： The method of chronic stress stimulation combined with bound was used to establish IBS model rats, then the methods of immunohistochemistry, enzyme-linked immunosorbent assay （ELISA） and reverse transcription polymerase chain reaction （ RT - PCR） were used to detect the expression of the lung tissue SP. Results ： Compared with those of control group, SP content of the lung tissue and the gene expression of IBS model rats increased significantly （ P 〈 0.01 ）. Conclusion： Under IBS condition, SP content of the lung tissue and gene expression were regulated upward, suggesting that the lung and large intestine are intrinsically linked through the nerve-endocrine system.

关 键 词：肠易激综合征 SP 基因表达 大鼠 

分 类 号：R574.4[医药卫生—消化系统]