柑橘黄酮体外清除活性氧自由基作用的研究  

Study on the Role in Scavenging Reactive Oxygen Free Radicals of Citrus Flavonoids in vitro

作　　者：陈世彪[1] 李莉[1] 李海涛[1] 

机构地区：[1]青海大学农牧学院,青海西宁810003

出　　处：《安徽农业科学》2010年第9期4553-4554,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究柑橘黄酮体外清除活性氧自由基的作用。[方法]加入柑橘黄酮后,测定邻苯三酚自氧化速率,研究柑橘黄酮体外清除活性氧自由基的作用,并与维生素C、75%乙醇的作用进行比较。[结果]0.1ml1mg/ml维生素C与0.1ml1mg/ml柑橘黄酮对45mmol/L邻苯三酚自氧化的平均抑制率分别达到50.98%±13.9%、27.78%±2.58%,二者间有极显著差异(P<0.01)。0.1ml75%乙醇对45mmol/L邻苯三酚自氧化的平均抑制率达到10.89%±1.84%,与0.1ml1mg/ml柑橘黄酮和0.1ml1mg/ml维生素C相比,有极显著差异(P<0.01)。[结论]柑橘黄酮具有显著地清除体外活性氧自由基的作用。[Objective] The paper aimed to study the role in scavenging reactive oxygen free radicals of Citrus flavonoids in vitro. [Method] The rate of Pyrogallol autoxidation was determined after adding Citrus flavonoids,the role inscavenging reactive oxygen free radicals of Citrus flavonoids in vitro was studied,and compared with the roles of vitamin C and 75% alcohol. [Result] The average inhibition rates of 0.1 ml 1 mg/ml vitamin C and 0.1 ml 1 mg/ml Citrus flavonoids to 45 mmol/L Pyrogallol autoxidation reached 50.98%±13.9%、27.78%±2.58%,there existed extremely difference （P0.01）. The average inhibition rate of 0.1 ml 75% alcohol to 45 mmol/L Pyrogallol autoxidation reached 10.89%±1.84%,compared with 0.1 ml 1 mg/ml Citrus flavonoids and 0.1 ml 1 mg/ml vitamin C,there existed extremely difference （P0.01）. [Conclusion] Citrus flavonoids played an obviously role in scavenging reactive oxygen free radicals of Citrus flavonoids in vitro.

关 键 词：邻苯三酚自氧化 活性氧自由基 维生素C 柑橘黄酮 

分 类 号：R284.1[医药卫生—中药学]