慢性宫颈炎组织内P_(53)突变的检测  

Determination of P 53 gene mutation in the tissue of chronic cervicitis 

作　　者：李文惠[1] 陈卫[1] 

机构地区：[1]兰州医学院生化教研室

出　　处：《兰州医学院学报》1998年第3期1-2,共2页Journal of Lanzhou Medical College

基　　金：甘肃省自然科学基金

摘　　要：目的：通过对慢性宫颈炎组织内Ｐ５３基因５－９外显子突变的研究，探讨慢性宫颈炎的发生是否与Ｐ５３的突变有关，并为临床妇科实验室建立一种分子生物学的基因检测方法。方法：应用ＰＣＲＳＳＣＰ技术，对慢性宫颈炎活检组织内Ｐ５３５－９外显子的突变进行了检测。结果：在１８例慢性宫颈炎组织中，仅有１例在ＰＣＲ后初步判断为可疑结果，但ＳＳＣＰ结果显示无突变，其余１７例ＰＣＲＳＳＣＰ检测结果均无Ｐ５３５－９外显子的改变。结论：慢性宫颈炎组织无Ｐ５３基因突变的现象，说明这种疾病的发生发展与Ｐ５３基因突变无关。Objective:It has been studied on in the basis of researching P 53 exon 5-9mutation the tissue of chronic cervicitis,this disease whether or not has something to do with mutation of P 53 gene and a gene determination method of molecular biology has been set up for clinical gynaecology laboratory.Method:By using PCR-SSCP technology determined mutations of P 53 exon 5-9 in the biopsy tissue of chronic cervicitis (noncancer).Results:Only the 1 case out of the 18 cases of chronic cervicitis was still dubious after PCR,but it did not mutate in SSCP.The others (17 cases) all did not determine mutation of P 53 exon 5-9 Conclusions:The phenomenon of mutation of P 53 exon 5-9 did not exist in the tissue of chronic cervicitis.The results showed clearly that occurrence and development of this disease has nothing to do with mutation of P 53 exon 5-9

关 键 词：慢性 宫颈炎 P53基因 PCR-SSCP 基因突变 

分 类 号：R711.320.4[医药卫生—妇产科学]