氯化锂-匹罗卡品致痫大鼠海马结构中CLC-2、CLC-3氯通道表达变化的研究  

Expressional changes of CLC-2 and CLC-3 chloride channel in the hlppocampus of rats with lithium-pilocarpine-induced epilepsy

作　　者：冯亚波[1] 姚红[2] 迟兆富[3] 满晓[1] 杜怡峰[1] 庞在英[1] 冷振璞[1] 

机构地区：[1]山东大学附属省立医院神经内科,济南250021 [2]山东大学附属省立医院保健神经科,济南250021 [3]山东大学齐鲁医院神经内科,济南250012

出　　处：《中华神经医学杂志》2010年第3期222-226,共5页Chinese Journal of Neuromedicine

基　　金：基金项目：山东省卫生厅计划项目（2007HZ066）

摘　　要：目的研究CIC-2、CIC-3氯通道在氯化锂一匹罗卡品大鼠慢性癫痫模型中分布和表达的变化，探讨其在癫痫发作病理机制中的作用。方法Wistar大鼠采用随机数字表法分成致痫组（60只）与对照组（20只），其中致痫组根据处死及处理时间又分为24h组、14d组与30d组，每组20只。致痫组复制氯化锂-匹罗卡品大鼠慢性癫痫模型，在发作后24h、14d、30d时，分别予以：（1）免疫组化染色，观察CIC-2、C1C-3氯通道蛋白在海马表达的分布情况及其致痫后不同时点的吸光度似）值的变化；（2）RT—PCR，观察C1C-2、C1C-3氯通道mRNA在致痫后不同时点的变化。结果（1）与对照组比较，致痫后14d至30d，致痫组免疫反应阳性神经元数和A值明显减少和降低，差异有统计学意义（p〈0．05）；C1C-2mRNA表达降低，差异有统计学意义（P〈0．05）。（2）与对照组比较，致痫组致痫后24h，海马CA1、CA3及齿状回各层C1C-3免疫反应阳性神经元数和A值明显增加和升高，差异有统计学意义（P〈O．05）；C1C-3氯通道mRNA表达明显增加，差异有统计学意义fK0．05）。结论癫痫慢性期的发作和C1C-2氯通道的减少有关。Objective To investigate the distribution and expression changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat models with lithium-pilocarpine-induced chronic epileptic, and discuss the significance of these changes in epileptic pathogenesis. Methods Eighty Wistar rats were randomly divided into status epilepticus （SE, n=60） and control （n=20） groups and rats in the SE group were assigned to 3 subgroups according to the different sacrificed times （24 h, 14 d and 30 d）. Rat models with chronic epileptic in the SE group were induced by lithium-pilocarpine. Immunohistochemistry was employed to observe the changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat＇s hippoeampal formation different time points after seizure. The mRNA changes of chloride channel CLC-2 and CLC-3 at different time points after seizure were observed by RT-PCR. Results Compared with those in the control group, the quantity of CLC-2 immune positive neurons and the average optical density in the SE group decreased obviously 14 and 30 d after seizure; so was the mRNA expression of CLC-2 （P〈0.05）, Compared with the control group, the SE group showed obviously increased quantity of CLC-3 immune positive neurons and optical density at each area of the hippocampus 24 h after seizure; so was the mRNA expression ofCLC-3 （P〈0.05）. Conclusion Seizures at chronic phase have relation with the decreased expressions of CLC-2.

关 键 词：癫痫 C1C-2 CIC-3 海马 

分 类 号：R742.1[医药卫生—神经病学与精神病学]