大鼠糖尿病血糖波动模型的建立  

Establishment of blood glucose fluctuation diabetic rats model

作　　者：廖洁[1] 雷闽湘[1] 胡维[1] 

机构地区：[1]中南大学湘雅医院内分泌科,长沙410008

出　　处：《中国医师杂志》2010年第2期157-161,共5页Journal of Chinese Physician

摘　　要：目的探讨利用胰岛素建立大鼠糖尿病血糖波动模型。方法取60只SD大鼠随机分成正常组（n=20）和模型组（n=40），高糖高脂饲料喂养模型组SD大鼠6周后用小剂量链尿佐菌素（STZ）30mg／Kg腹腔注射诱导建立糖尿病模型，模型成功后将其随机分成持续高糖组（n=20）和血糖波动组（n=20）。血糖波动组大鼠每日2次皮下注射普通胰岛素诱导血糖波动，从而建立糖尿病大鼠血糖波动模型。观察大鼠“三多一少”症状，血糖变化情况。结果（1）血糖波动组和持续组的“三多一少”症状明显。（2）血糖波动组的SDBG，LAGE明显高于正常组[（8．56±0．49）mmol／LVS（0．69±0．05）mmol／L，（23．15±2．51）mmol／LVS（2．14±0．52）mmol／L]及持续组[（8．56±0．49）mmol／LV8（3．25±0．26）mmol／L，（23．15±2．51）retool／Lvs（9．45±2．46）mmol／L]，持续组的MBG（24．66±3．32）mmol／L高于正常组（6．10±0．75）mmol／L及波动组（18．16±8．91）mmol／L。（3）持续组和波动组的24h尿蛋白均较普通组明显增加[（100．38±7．99）mg,／24hVS（18．13±1．41）mg／24h，（123．71±11．23）mg／24h VS（18．13±1．41）mg／24h]，差异有统计学意义（P〈0．01），波动组较持续组增加[（123．71±11．23）mg／24hVS（100．38±7．99）mg／24h]，差异有统计学意义（P〈0．05）。结论使用每日皮下注射两次胰岛素的方法能成功制作出糖尿病大鼠血糖波动模型，并可作为研究血糖波动及其并发症的理想动物模型。Objective To establish the blood glucose fluctuation diabetic rat model by subcutaneous injecting insulin. Method The male Sprague-Dawley （SD） rats were randomly assigned into two groups, normal group （ n = 20） and model group （ n = 40）. Normal group rats were fed with normal diet, model group rats were fed with a high-sucrose-high-fat diet. After 6 weeks, a low dose of streptozotocin （STZ , 30mg/kg） was injected to induce hyperglycemia in model group rats. And then the model group rats were random divided into two subgroups, diabetic group （ n = 20） and blood glucose fluctuation group （ n = 20）. Blood glucose fluctuation group rats were induced by subcutaneous injecting insulin twice daily. All rats were executed after intervening for 3 months, metabolic cages were used to collect 24-hour urine one week before all rats were executed. Results The average blood glucose levels （ MBG）, the standard deviation of daily average blood glucose （SDBG） and the largest amplitude of glycemic excursions （LAGE） were significant different （ P 〈 0. 05 ） among normal group, diabetic group and blood glucose fluctuation group. The 24 hours pro- teinuria of diabetic group and blood glucose fluctuation group was higher than that in normal group[ （ 100. 38 ± 7.99） mg/24h vs （ 18. 13 ± 1.41 ） mg/24h, （ 123.71 ± 11.23 ） mg/24h vs （ 18. 13 ± 1.41 ） mg/24h, P 〈 0. 01], and blood glucose fluctuation group was higher than diabetic group[ （123.71 ± 11.23）mg/24h vs （100. 38 ± 7. 99）mg/24h, P 〈 0. 05 ]. Conclusions The blood glucose fluctuation diabetic rats model was successfully induced by the method of subcutaneous insulin injection in diabetic rats twice daily.

关 键 词：糖尿病 实验性 血糖 模型 动物 

分 类 号：R-332[医药卫生]