骨组织工程中脂肪间充质干细胞的作用  

Role of adipose-derived mesenchymal stem cells in bone tissue engineering

作　　者：贾经汉[1] 张志[1] 王向鹏[1] 邹冬青[1] 

机构地区：[1]广西中医学院附属瑞康医院骨科,广西壮族自治区南宁市530011

出　　处：《中国组织工程研究与临床康复》2010年第1期166-170,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前常采用在骨缺损处充填自体骨、异体骨、人工合成骨替代品等方法进行骨缺损修复,但临床效果均不理想。研究表明脂肪间充质干细胞具有多种分化能力及很强的增殖潜力,可将其诱导分化成骨。目的:分析脂肪间充质干细胞在骨组织工程中的应用情况,以明确脂肪间充质干细胞是否可以作为骨组织工程中的种子细胞。方法:应用计算机检索Medline数据库(1999-01/2008-12),以adipose tissue-derived mesenchymal stem cells,adipose mesenchymal stem cells,adipose stem cell;osteogenic induction,osteogenic inducement,bone induction,osteoblastic induced;chondroblast induction,cartilage induction;bone tissue engineering,tissue engineering bone,tissue engineering of bone为检索词;应用计算机检索清华同方数据库(2003-01/2008-12),以脂肪间充质干细胞、成骨诱导、成软骨诱导、骨组织工程为检索词。结果与结论:共收集361篇关于脂肪间充质干细胞与骨组织工程相关的文献,中文246篇,英文115篇。排除发表时间较早、重复及类似研究,纳入29篇符合标准的文献。脂肪间充质干细胞体外扩增和自我更新能力很强,传代培养易于获得大量有分化能力的细胞,是真正意义上的具有多向分化潜能的干细胞,脂肪间充质干细胞在一定的培养条件下,可定向分化为成骨细胞、软骨细胞及骨细胞和肌肉细胞,配合合适的支架后可以应用到骨组织工程中,可以作为骨组织工程中的种子细胞。BACKGROUND： At present, bone defects usually repaired by autologous bone, allogenic bone, synthetic bone substitutes and other methods, which received poor clinical results. Preliminary studies have shown that adipose-derived mesenchymal stem cells （ADSCs） possess strong proliferation ability and differentiation potential, and can be induced differentiate into bone. OBJECTIVE： To analyze the application of ADSCs in bone tissue engineering, and to identify whether ADSCs can be used as seed cells in bone tissue engineering. METHODS： The databases of PubMed （1999-01/2008-12） and Tongfang （2003-01/2008-12） was retrieved using key words of ＂adipose tissue-derived mesenchymal stem cells, adipose mesenchymal stem cells, adipose stem cell; osteogenic induction, osteogenic inducement, bone induction, osteoblastic induced; chondroblast induction, cartilage induction; bone tissue engineering, tissue engineering bone, tissue engineering of bone＂ . RESULTS AND CONCLUSION： A total of 361 literatures were collected, including 246 in Chinese and 115 in English. Totally 29 literatures were accordant with the study criteria. ADSCs is a truly multi-directional differentiation potential cells, which possess strong amplification and self-renewal potential, and can be directional differentiated into osteoblasts, cartilage cells, bone cells and muscle cells. It can be used as seed cells in bone tissue engineering when matching appropriate stents.

关 键 词：脂肪间充质干细胞 成骨诱导 成软骨诱导 骨组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]