构建腭中缝牵张实验大鼠动物模型  

Establishment of a rat midpalatal suture expansion model

作　　者：郭婧[1] 张奇峰[2] 李贵凤[1] 刘泽萍[1] 周静[1] 邹淑娟[1] 

机构地区：[1]口腔疾病国家重点实验室,四川大学华西口腔医院正畸科,四川省成都市610041 [2]浙江大学医学院附属第一医院口腔医疗中心,浙江省杭州市310003

出　　处：《中国组织工程研究与临床康复》2010年第2期272-275,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30772454);四川省科技厅攻关项目(2006z09-013)~~

摘　　要：背景:以往曾采用猴、犬、猪、兔等大动物作为腭中缝牵张动物模型,但存在费用高,样本量小,检测抗体难获得等缺点。Wistar大鼠头部较宽阔,便于腔方面的操作,且成本低,繁殖率高,作为腭中缝牵张模型,有可能克服以上缺陷。目的:建立大鼠腭中缝牵张实验动物模型,为进一步进行腭中缝牵张的实验动物研究提供基础。方法:5周龄Wistar雄性大鼠20只,平均体质量65g,随机分成实验组与对照组,每组10只。实验组采用0.014澳丝弯制双眼圈簧样扩弓装置,插入第一、二磨牙牙间隙,用光固化树脂粘结在大鼠磨牙舌侧进行固位;对照组安装未加力的同型扩弓装置。主动加力1周。在扩弓结束后分别对大鼠腭中缝进行X射线片,组织学观察。结果与结论:上颌骨X射线片发现实验组腭中缝明显扩宽,磨牙明显颊向倾斜。经光镜观察发现,实验组腭中缝偏口腔侧明显增宽,间充质细胞呈梭性,与牵张力方向一致。在其下方出现创伤性炎症反应,有明显的出血区。结果证实,实验大鼠腭中缝牵张动物模型满足实验研究的需要,具有操作简单、设计科学、可重复性强的特点。BACKGROUND：Monkeys,dogs,pigs,rabbits and other large animals have been applied previously to prepare animal models of midpalatal suture expansion,but there are high cost,small sample size,difficult to obtain antibodies and other disadvantages. Wistar rats have wide heads to facilitate cavity operation,with low cost and high reproduction rate,as the midpalatal suture model,it is possible to overcome the above deficiencies. OBJECTIVE：To establish a rat model of midpalatal suture expansion,and to supply basement for further relative researches of animal models. METHODS：Twenty Wistar male rats of 5 weeks old,average weight of 65 g,were randomly divided into 2 groups,a experiment and a control,with 10 rats in each group. Rats in experimental group were placed on the expansion appliance,inserting into the diastema between the first and second molars,then stick to molar lingual using light-cured resin for retention. The rats in control group were sham operated,followed by one week of active expansion. After expansion,the midpalatal sections were observed by X-ray and light microscopy. RESULTS AND CONCLUSION：Maxillary X-ray film showed that midpalatal suture in experimental group was significantly widened,molar lead to cheek. Observed by light microscopy,partial oral side of midpalatal suture in the experimental group was obviously enlarged,mesenchymal cells were spindle,in the same direction to tension force. Below it,traumatic inflammatory response appeared,with a clear bleeding area. The midpalatal suture expansion model in rats is available,simple and reproducible.

关 键 词：上颌快速扩弓 腭中缝牵张:大鼠 动物模型 口腔组织工程 

分 类 号：R-332[医药卫生]