西安地区宿主IL-1β基因多态性与胃癌的相关性研究  

The relationship between Interleukin-1 beta gene polymorphisms and gastric cancer in Xi'an area

作　　者：乔文[1] 李娜 李长顺[1] 

机构地区：[1]西安交通大学医学院第一附属医院消化科,陕西西安710061 [2]兵器工业部521医院消化科

出　　处：《胃肠病学和肝病学杂志》2010年第3期205-208,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨胃癌高发的西安地区宿主IL-1β基因多态性与胃癌发生的相关性。方法70例胃癌和70例慢性胃炎胃黏膜组织,均取自西安交通大学医学院第一附属医院内镜活检标本和手术切除标本,提取组织DNA,采用PCR-RFLP方法进行IL-1β基因-31位点、-511位点多态性的检测。结果IL-1β-31位点上有三种基因型即C/C、T/T和C/T。胃癌组与慢性胃炎组IL-1β-31位点上基因型比较,胃癌组携带-31C/-31T基因型的几率是慢性胃炎组的2.940倍,有显著性差异(P<0.05);-31T/-31T两者无显著性差异(P>0.05)。携带IL-1β-31T等位基因者患胃癌的风险性增加3.000倍。IL-1β-511位点上也有三种基因型即C/C、T/T和C/T。胃癌组与慢性胃炎组IL-1β-511位点上基因型比较,胃癌组携带-511C/-511T和-511T/-511T基因型的几率是慢性胃炎组的3.068倍和4.500倍,有显著性差异(P<0.05)。携带IL-1β-511T等位基因者患胃癌的风险性增加3.574倍。结论西安地区胃癌患者IL-1β-31T和IL-1β-511T基因携带频率高,且-511T/-511T患胃癌的风险性高,表明携带IL-1β-511T等位基因,尤其是-511T/-511T纯合子,可能是胃癌发生中的宿主易感因素。Objective To study the relationship between Interleukin-1beta（IL-1β） gene polymorphisms and gastric cancer in Xi＇an area.Methods Gastric tissues from 70 gastric cancer and 70 chronic gastritis patients in Xi＇an area were collected.The genomic DNA was extracted from the gastric tissues.The IL-1β polymorphisms were analyzed by PCR-restriction fragment length polymorphism（PCR-RFLP）.Results There were three genotypes in IL-1β-31 locus.They were C/C,T/T and C/T.Compared with the chronic gastritis,-31C/-31T in gastric cancer was found to have higher frequency,the risk increased 2.940 times（P0.05）;-31T/-31T frequencies were similar between gastric cancer and chronic gastritis（P0.05）.IL-1β-31T allele frequencies in gastric cancer and chronic gastritis were 42.85% and 20.00%,respectively.The risk to be gastric cancer increased 3.000 times.There were three genotypes in IL-1β-511 locus,too.They were C/C,T/T and C/T.Compared with chronic gastritis,-511C/-511T and-511T/-511T in gastric cancer were found the risk increased 3.068 times and 4.500 times（P0.05）;IL-1β-511T allele frequencies in gastric cancer and chronic gastritis were 77.14% and 48.57%,respectively.The risk to be gastric cancer increased 3.574 times.Conclusion The carrying T genotype frequencies in IL-1β-31T/-511T of gastric cancer were higher than gastritis in Xi＇an area,especially-511T/-511T had the high risk.These suggested IL-1β-511T allele may be the infected factor of host in the occurrence of gastric cancer.

关 键 词：白细胞介素1Β 基因多态性 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]