高效液相色谱法测定皮下植入顺铂的血浆及肾脏浓度  

High-performance liquid chromatography-based determination of plasma and renal tissue cisplatin levels after subcutaneous cisplatin implantation in mice

作　　者：李维凤[1] 牛晓峰[1] 范特[1] 王小鹏[2] 刘燕玲[1] 陈天宁[2] 

机构地区：[1]西安交通大学医学院,陕西西安710061 [2]西安交通大学机械工程学院,陕西西安710049

出　　处：《南方医科大学学报》2010年第2期242-244,共3页Journal of Southern Medical University

基　　金：国家863计划项目(2006AA04Z330);国家自然科学基金(50705074)

摘　　要：目的建立高效液相色谱测定皮下植入顺铂血浆和肾脏中顺铂(DDP)浓度的方法。方法采用高效液相色谱法测定血浆和组织中的顺铂经二乙基二硫代氨基甲酸钠(DDTC)络合衍生化后用乙醚提取的产物Pt(DDTC)2。采用岛津LC-2010A高效液相色谱仪,流动相水-甲醇(25:75),流速1.0ml/min,在254nm处测定药物浓度。结果顺铂的线性范围为0.1～10μg/ml,r=0.9998(血浆),r=0.9993(肾脏),日内RSD和日间RSD均小于10%,最低检测限为50ng。结论本法是一种准确有效的检测方法,适用于皮下植入顺铂的血药浓度监测和药动学研究。Objective To develop a method for determining plasma and renal tissue concentrations of cisplatin（DDP） after subcutaneous DDP implantation in mice.Methods DDP was extracted from the plasma and tissue of mice receiving subcutaneous DDP implantation and reacted with sodium diethyldithiocarbamate（DDTC）.The product Pt（DDTC）2 extracted by diethyl ether was determined using high-performance liquid chromatography（HPLC） with the mobile phase of water and methanol at the ratio of 25：75 and the flow rate of 1.0 ml/min.The derivatives of DDP and nickel chloride were detected at the wavelength of 254 nm.Results The linear range of DDP was 0.1-10 μg/ml（r=0.9998 for plasma and 0.9993 for kidney）.The intra-day and inter-day RSD was below 10%,and the minimum concentration detectable was 50 ng.Conclusion The method is accurate and effective for determining plasma and tissue DDP levels after subcutaneous DDP administration and can be used in pharmacokinetic study of DDP.

关 键 词：高效液相色谱法 顺铂 皮下植入 血浆 肾 DDTC 

分 类 号：R73-3[医药卫生—肿瘤]