Ad-VEGF-GFP-CD/TK双自杀基因系统对大肠癌的生长抑制作用及微环境中细胞因子活性的影响  

Effect of adenovirus-mediated CD/TK double suicide gene system on colorectal cancer growth and cytokines in the tumor microenvironment in mice

作　　者：姚晓军[1] 黄宗海[1] 李强[1] 王朝阳[1] 

机构地区：[1]南方医科大学珠江医院普通外科,广东广州510282

出　　处：《南方医科大学学报》2010年第2期260-262,共3页Journal of Southern Medical University

基　　金：国家863计划项目(2001AA217171)

摘　　要：目的评价重组腺病毒介导的Ad-VEGF-GFP-CD/TK双自杀基因系统对大肠癌Balb/c小鼠移植瘤的治疗作用及其引起的相关细胞因子的变化。方法Balb/c小鼠皮下接种CT26细胞,成瘤后给予双自杀基因系统治疗,计算肿瘤体积抑瘤率的动态变化,治疗结束后检测IL-2、IL-10、TNFα、IFNγ这4种细胞因子的表达水平。结果CD/TK双自杀基因系统对大肠癌的生长抑制作用明显;治疗后小鼠4种细胞因子水平均有升高,与对照组有显著差异(P<0.05)。结论重组腺病毒介导的CD/TK双自杀基因系统对荷大肠癌Balb/c小鼠的移植瘤有明显的抑瘤作用,其所导致的细胞因子的变化可能有助于加强其抗肿瘤效应。Objective To evaluate the effect of adenovirus-mediated CD/TK double suicide gene system on tumor growth and cytokine levels in the tumor microenvironment in mice bearing transplanted colorectal cancer.Methods CT26 cells were implanted subcutaneously into 30 Balb/c mice,which were subsequently randomized into the control（n=15） and experimental group（n=15）.After the tumor formation,CD/TK double suicide gene system was administered for tumor treatment,and the changes in the tumor volume,tumor inhibition rate,and levels of cytokines in the tumor microenvironment were investigated.Results CD/TK double suicide gene system resulted in a significant inhibition of the tumor growth and significantly increased levels of such cytokines as IL-2,IL-10,TNFα and IFNγ in the tumor microenvironment.Conclusion CD/TK double suicide gene system produces significant tumor inhibition effect and causes obvious cytokine changes in the tumor microenvironment in mice bearing transplanted colorectal cancer.

关 键 词：自杀基因疗法 大肠癌 细胞因子 

分 类 号：R735.34[医药卫生—肿瘤]