黄体酮促进培养乳腺癌细胞的增殖和迁移  

Progesterone promotes the proliferation and migration of cultured breast cancer cells

作　　者：赵嘉佳[1] 王辛[2] 霍中军[1] 罗深秋[1] 熊静波[1] 

机构地区：[1]南方医科大学细胞生物学教研室,广东广州510515 [2]南方医科大学病理学教研室,广东广州510515

出　　处：《南方医科大学学报》2010年第3期443-446,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30470673);广东省自然科学基金(4020364);教育部留学回国人员启动基金(教外司留[2004]176号)

摘　　要：目的研究黄体酮对乳腺癌细胞增殖和迁移的影响。方法100nM黄体酮刺激黄体酮受体阳性的乳腺癌细胞株T-47D和MCF-748小时后,用MTT方法检测细胞增殖,用细胞划痕愈合实验检测细胞迁移,用Western blotting检测细胞内E-Cadherin的表达。结果黄体酮刺激MCF-7和T-47D细胞的增殖和迁移;并且可以下调MCF-7和T-47DE-Cadherin的表达。结论黄体酮能够促进培养乳腺癌细胞的增殖和迁移,提示抗黄体酮治疗可能有临床意义。Objective To investigate the effects of progesterone on the growth and migration of breast cancer cells. Methods MCF-7 and T-47D cells were cultured in DMEM and stimulated with 100 nmol/L progesterone for 48 h, and the cell proliferation was evaluated by MTT assay, cell migration by wound-healing assay and E-catherin expression by Western blotting. Results Progesterone stimulated the cell proliferation and migration and down-regulated the expression of E-catherin in both MCF-7 and T-47D cells. Conclusions Progesterone stimulates the cell proliferation and migration of cultured breast cancer cells, suggesting the clinical significance of anti-progesterone therapy in breast cancer.

关 键 词：黄体酮 乳腺癌 细胞增殖 细胞迁移 

分 类 号：R737.9[医药卫生—肿瘤]