EB病毒Rta/IgG、EBNA1/IgA、VCA/IgA及EA/IgA抗体与鼻咽癌分期的关系  

Relationship between clinical stages of nasopharyngeal carcinoma and Epstein-Barr virus antibodies Rta/IgG, EBNA1/IgA, VCA/IgA and EA/IgA

作　　者：蔡永林[1,2] 郑裕明[2] 成积儒 王伟[3] 张旖旎[3] 王卫华[4] 吴英松[1] 杨淑君 钟伟铭 李军[2] 莫永坤 

机构地区：[1]南方医科大学生物技术学院,广东广州510515 [2]广西梧州市红十字会医院,广东梧州543002 [3]南方医科大学第一临床医学院,广东广州510515 [4]湖北大学数计算学院,湖北武汉430062 [5]广州军区海军421医院特检科,广东广州510310

出　　处：《南方医科大学学报》2010年第3期509-511,共3页Journal of Southern Medical University

基　　金：广西壮族自治区卫生厅计划课题(Z2008472);梧州市科技计划项目(200801063)

摘　　要：目的探讨EB病毒Rta/IgG、EBNA1/IgA、VCA/IgA及EA/IgA抗体与鼻咽癌临床分期的关系。方法收集211例未经治疗的鼻咽癌患者的血清,用酶联免疫吸附法(ELISA)检测Rta/IgG、EBNA1/IgA抗体,用免疫酶法检测VCA/IgA及EA/IgA抗体,按"92分期法"进行分期,分别计算各T、N、M分期及临床分期的各抗体阳性率及抗体水平并进行统计学分析。结果鼻咽癌各T、N、M分期及临床分期组Rta/IgG抗体rA值均无统计学差异(P>0.05)。T1期的EBNA1/IgA抗体rA值明显低于其它T分期,N0期明显低于其它N分期,临床I期明显低于其它临床分期(P<0.05)。VCA/IgA、EA/IgA抗体滴度各N分期及临床分期间比较,差异有统计学意义(P<0.05)。结论EB病毒Rta/IgG抗体表达与鼻咽癌分期无关。EBNA1/IgA抗体在早期鼻咽癌表达水平相对较低。而VCA/IgA、EA/IgA抗体水平与颈部淋巴结转移程度相关,对于评估鼻咽癌临床分期有一定的参考价值。Objective To evaluate the relationship between the clinical stages of nasopharyngeal carcinoma （NPC） and Epstein-Barr virus （EBV） antibodies Rta/IgG, EBNA1/IgA, VCA/IgA and EA/IgA. Methods Serum samples obtained from 211 untreated patients with NPC categorized by the project of 92＇ stage were examined for the presence of the EBV antibodies Rta/IgG and EBNA1/IgA by enzyme-linked immnunosorbent assay （ELISA） and for VCA/IgA and EA/IgA by immunoenzymatic assay. The positive rates and antibody levels in the NPC patients in different TNM stages and clinical stages were analyzed statistically. Results No significant difference in Rta/IgG rA value was found in the NPC patients in different TNM or clinical stages （P0.05）. The EBNA1/IgA rA value was significantly lower in stage T1, N0, and clinical stage I than in the other corresponding T stages, N stages and other clinical stage （P0.05）. The antibody titers of VCA/IgA and EA/IgA differed significantly between the N stages and the clinical stages （P0.05）. Conclusion The expression of EBV Rta/IgG is not associated with NPC stage. The expression of EBNA1/IgA is relatively low in early NPC. The antibody level of VCA/IgA and EA/IgA are significantly correlated to the degree of neck lymph node metastasis, and might be helpful to classify the clinical stages of NPC.

关 键 词：鼻咽癌 EB病毒 抗体 临床分期 

分 类 号：R739.63[医药卫生—肿瘤]