重组人促红细胞生成素对急性高眼压兔视网膜bcl-2蛋白表达的影响  

Effect of recombinant human erythropoietin on bcl-2 protein expression in the retina in a rabbit model of acute high intraocular pressure

作　　者：王建明[1] 宋艳萍[2] 孙乃学[1] 惠娜[1] 赵世平[1] 胡凯[2] 

机构地区：[1]西安交通大学医学院第二附属医院眼科,陕西西安710004 [2]西安市中心医院血液科,陕西西安710003

出　　处：《南方医科大学学报》2010年第3期552-554,共3页Journal of Southern Medical University

基　　金：陕西省科技攻关资助项目(2008K14-07);西安市社发医药卫生研究资助项目(SF08004-2)

摘　　要：目的探讨全身应用重组人促红细胞生成素(rhEPO)对急性高眼压兔眼视网膜bcl-2蛋白表达的影响及意义。方法在设立正常对照、急性高眼压模型对照的情况下,日本大耳白兔皮下注射rhEPO,分别于造模后第1、3、7、14天免疫组化法观察视网膜bcl-2蛋白表达。结果正常视网膜组织可见bcl-2蛋白表达,染色阳性细胞数为每高倍视野(10.5±1.2)个。模型组和EPO组兔眼视网膜bcl-2蛋白表达阳性细胞数均较正常对照组减少(P<0.05,P<0.01)。EPO组第7天、14天视网膜bcl-2蛋白表达阳性细胞数较模型组增加(P<0.05,P<0.01)。结论rhEPO可以上调视网膜bcl-2蛋白表达,这可能是rhEPO保护急性高眼压引起的兔眼视网膜神经功能损害的机制之一。Objective To investigate the effect of recombinant human erythropoietin （rhEPO） on the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure and explore the mechanism underlying the protective effect of rhEPO on the retina against ischemia-reperfusion injury. Methods rhEPO was injected subcutaneously in the ear of a rabbit model of acute high intraocular pressure induced by physiological saline perfusion into the anterior chamber. Bcl-2 protein expression in the retina of the rabbits was observed by immunohistochemical staining on days 1, 3, 7, and 14 after retinal ischemia-reperfusion and compared with that in normal rabbits and untreated rabbit models. Results bcl-2-positive cells were observed in the retina of normal rabbits with a mean positive cell number of 10.5 ±1.2 in each high-power visual field. Compared with that in the normal control group, the number of the positive cells decreased significantly in both the model group and EPO group （P0.05, P0.01）, but the latter group showed a significantly greater number than the former （P0.05 at day 7 and P0.01 at day 14）. Conclusion Systemic administration of rhEPO can up-regulate the expression of bcl-2 protein in the retina of rabbits with acute high intraocular pressure, which is probably one of the mechanisms for the protective effect of rhEPO on the retina against ischemia-reperfusion injury.

关 键 词：促红细胞生成素 BCL-2蛋白 视网膜 神经保护 

分 类 号：R775.9[医药卫生—眼科]