大鼠血浆中阿托品的LC-M/SMS测定  

Determination of atropine in rat plasma by LC-MS/MS

作　　者：朱加银[1] 程晶晶[2] 王贤亲[3] 李翔[1] 陈锡文[1] 

机构地区：[1]温州医学院实验动物中心,浙江温州325035 [2]温州医学院附属口腔医院,浙江温州325000 [3]温州医学院分析测试中心,浙江温州325035

出　　处：《中国卫生检验杂志》2010年第3期516-518,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立LC-MS/MS法测定大鼠血浆中的阿托品。方法：血浆样品经乙腈沉淀蛋白提取。采用C18色谱柱,以乙腈-0.1%甲酸（60：40,v/v）为流动相,氯氮平为内标,采用电喷雾电离源（ESI）,选择正离子多离子反应监测（MRM）,质核比（m/z）为290.0→123.9（阿托品）和m/z 327.1→269.9（氯氮平）。结果：阿托品在5~1000 ng/ml浓度范围内线性关系良好。方法回收率大于97%,提取回收率大于85%,日内和日间RSD小于8%。结论：方法简便、快速、准确,可满足临床药动学研究的要求。Objective：A LC-MS/MS method was established for the determination of atropine in rat plasma.Method：Plasma samples were treated with acetonitrile to deposit proteins.A C18 column was used with the mobile phase of acetonitrile-0.1%formic acid（60：40,v/v）.Clozapine was used as internal standard.ESI was applied in positive ion mode.Multiple reaction monitoring（MRM）mode was used.The detected ions were m/z 290.0→123.9 for atropine and m/z 327.1→269.9 for clozapine.Results：The calibration curve was linear in the concentration of 5~1000 ng/ml.The average recovery was more than 97% and the average extraction recovery was above 85%.The inter-and intra-day RSD were less than 8%.Conclusion：The method is simple,convenient and accurate,which may be suitable for the pharmacokinetic study of atropine.

关 键 词：阿托品 液相色谱-串联质谱 测定 氯氮平 血浆样品 大鼠 回收率 蛋白提取 浓度范围 电喷雾电离源 

分 类 号：O657.72[理学—分析化学]