亚胺培南对肺炎克雷伯菌抑菌圈直径与KPC型碳青霉烯酶检测  

Imipenem inhibition zone and KPC type carbapenemase in Klebsiella pneumoniae

作　　者：赵建萍[1] 黄金伟[1] 陈丽珠[1] 李爱芳[1] 黄建胜[1] 王晓光[2] 陈秀英[2] 李国雄[1] 

机构地区：[1]浙江省丽水市中心医院检验科,浙江丽水323000 [2]浙江省丽水市疾病预防控制中心,浙江丽水323000

出　　处：《中国卫生检验杂志》2010年第3期567-569,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨临床分离的肺炎克雷伯菌的亚胺培南抑菌圈直径大小与KPC型碳青霉烯酶（Carbapenemase KPC）存在的相关性。方法：利用ESBLs确认试验对临床分离的肺炎克雷伯菌产ESBLs检测,并分为ESBLs阳性组与ESBLs阴性组,K-B法检测亚胺培南对所有菌抑菌圈直径,PCR方法检测所有菌KPC。结果：亚胺培南对81株肺炎克雷伯菌ESBLs阳性组抑菌圈直径27.92±0.18 cm,统计学方法推论95%抑菌圈范围27.10～28.48 cm;ESBLs阴性组101株抑菌圈直径28.28±0.11 cm,统计学方法推论95%抑菌圈范围27.40～28.86 cm,2组抑菌环直径比较无明显差异（P0.05）。2株ESBLs阳性肺炎克雷伯菌亚胺培南抑菌圈直径分别为20 cm和19.5 cm,同时检测到KPC。结论：亚胺培南对临床分离的肺炎克雷伯菌抑菌圈直径小于或等于20 cm,该菌检测到KPC型碳青霉烯酶可能是原因之一。Objective：To analyze the relationship between imipenem inhibition zone and KPC type Carbapenemase in Klebsiella pneumoniae.Methods：K.pneumoniae strains were separated in two groups according to ESBLs.Imipenem inhibition zone were measured with disc dilution method,while KPC gene was detected by PCR.Results：Imipenem inhibition zone in 81 ESBLs positive and 101 ESBLs negative strains were 27.92±0.18 cm and 28.28±0.11 cm,respectively,according to statistical method.But there was no significant discrepancy between the two groups（P0.05）.KPC gene were detected in 2 ESBLs positive strains with the inhibition zone of 20 cm and 19.5 cm respectively.Conclusion：KPC type Carbapenemase may contribute to imipenem resistant in K.pneumoniae with the inhibition zone = 20 cm.

关 键 词：克雷伯菌 肺炎 亚胺培南 KPC 抑菌圈直径 碳青霉烯类抗生素 统计学方法 相关性 酶检测 阳性 

分 类 号：R446.5[医药卫生—诊断学]