siRNA对人食管鳞癌EC9706细胞中桩蛋白表达的影响  

Effect of siRNA on the expression of paxillin in human esophageal carcinoma EC9706 cells

作　　者：高冬玲[1] 郭新玲[2] 陈明勇[1] 李晟磊[1] 张云汉[1] 陈奎生[1] 

机构地区：[1]郑州大学第一附属医院病理科．河南省肿瘤病理重点实验室,450052 [2]新乡医学院第三附属医院麻醉科,453003

出　　处：《实用医学杂志》2010年第5期721-723,共3页The Journal of Practical Medicine

基　　金：河南省基础与前沿技术研究基金资助项目(编号:082300453202)

摘　　要：目的:探讨小分子干扰RNA(siRNA)对人食管鳞癌EC9706细胞中桩蛋白(paxillin)表达的抑制作用。方法:使用paxillin siRNA转染人食管鳞癌EC9706细胞,以对照siRNA转染组和正常EC9706细胞组为对照,采用免疫细胞化学SP法、Western blot和半定量RT-PCR技术分别检测转染前后上述各组细胞中paxillin蛋白和mRNA的表达。结果:转染paxillin siRNA后,免疫细胞化学结果显示,3组细胞中paxillin siRNA转染组中paxillin蛋白阳性表达细胞数最低,组间比较差异具有统计学意义(P<0.05);Western blot及RT-PCR结果显示,与正常EC9706细胞组及对照siRNA转染组相比,paxillin siRNA转染组中的paxillin蛋白及mRNA的表达量明显下调,差异有统计学意义(P<0.05)。结论:paxillin siRNA能有效抑制人食管鳞癌EC9706细胞中paxillin基因的表达。Objective To investigate inhibitory effect of small interfering RNA （siRNA） on expression of paxillin gene in human esophageal carcinoma EC9706 cells. Methods Paxillin-siRNA was used to transfect EC9706 cells, normal EC9706 cells group and transfected with control siRNA group were situated as control. Expressions of paxillin protein and mRNA before and after transfection were detected using immunocytochemistry, Western blot and semiquantitative RT-PCR respectively. Results After the transfection of paxillin, results of immunocytochemistry demonstrated that positive cell numbers of paxillin protein were lowest in the paxillin siRNA transfection group among the three groups （P 〈 0.05） ; positive cell numbers of normal EC9706 cell group and control siRNA transfection group were higher than that of paxillin siRNA transfection group （P 〈 0.05）, there was no difference between the two control groups （P 〉 0.05）. Results of Western blot and Semi-quantitative RT-PCR demonstrated that compared with normal EC9706 cell gruap and control siRNA transfection group, expression levels of paxillin protein and mRNA were obviously down-regulated after transfection with paxillin siRNA, the difference has statistical significance （P 〈 0.05 ）. Conclusion Specific paxillin-siRNA can effectively inhibit the expression of paxillin gene in human esophageal carcinoma EC9706 cells.

关 键 词：食管肿瘤 SIRNA EC9706细胞 桩蛋白 免疫细胞化学 聚合酶链反应 蛋白印迹 

分 类 号：R735.1[医药卫生—肿瘤]