加巴喷丁联合吗啡对神经病理性疼痛家兔影响的实验观察  

Effects of Gabapentin combined with Morphine on neuropathic pain of rabbit

作　　者：欧阳婉[1] 梁锐[2] 黄冰[2] 吴裕超[2] 刘悦[2] 孟志秀[2] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学肿瘤医院麻醉科,南宁530021

出　　处：《中国癌症防治杂志》2010年第1期1-4,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西自然科学基金资助项目(桂科自0640156)

摘　　要：目的观察加巴喷丁联合吗啡对神经病理性疼痛家兔的镇痛作用及对炎性细胞因子TNF-α、IL-6的影响。方法对25只家兔进行坐骨神经结扎并建立神经病理性疼痛模型。1周后选取建模成功的20只家兔随机分为4组:生理盐水组(NS)、吗啡组(M)、加巴喷丁组(G)和加巴喷丁联合吗啡组(MG),分别给予相应的药物处理。观察给药前后家兔的行为学改变,并测定给药前后神经递质5-HT、NE和炎性细胞因子TNF-α、IL-6表达的变化。结果①家兔的行为学改变:给药后M、G、MG3组家兔自发痛都有不同程度的改善,MG组早于G组,G组早于M组,NS组则无明显改变。②神经递质改变:给药后M、G、MG3组均低于治疗前(P<0.05),且MG组降低早于M组和G组,NS组则无明显改变。③炎性细胞因子的变化:给药后M、G、MG3组均低于治疗前,且MG组降低早于M组、G组,NS组则无明显降低。结论加巴喷丁联合吗啡对神经病理性疼痛家兔具有较好的协同镇痛作用,优于单用吗啡和加巴喷丁,同时能够降低炎性细胞因子TNF-α、IL-6的表达。Objective To investigate the analgesic effect of Gabapentin combined with Morphine on neuropathic pain of rabbit,and the effects on inflammatory cytokines TNF and IL-6.Methods Twenty rabbits with successfully established neuropathic pain were randomly divided into four groups：saline（NS）,Gabapentin（G）,Morphine（M） and combination of Gabapentin and Morphine（MG）.Animals in each group were given the appropriate drug by gastric gavage.Before and after administration,the behavioral changes of the rabbits were observed,while the neurotransmitters 5-HT and NE,inflammatory cytokines TNF-α and IL-6 in serum were measured by ELISA.Result 1.Behavioral change：after administration,the spontaneous pain of rabbits in groups M,G and MG had various degree of improvement,which appeared earliest in MG group,while the G group appeared earlier than M group.The NS group had no significant change.2.Neurotransmitter change：the neurotransmitters in serum reduced in Groups G,M and MG after administration（P0.05）,which appeared earlier in group MG than that in group M and G.Group NS had no significant change.3.Inflammatory cytokines change：after administration,the inflammatory cytokines in serum decreased in groups G,M and MG,which appeared earlier in group MG than that in group M and G.Group NS had no significant change.Conclusions The synergistic effect of Gabapentin and Morphine can effectively relieve neuropathic pain in rabbits,and can reduce the expressions of inflammatory cytokine TNF and IL-6.The analgesic efficacy of Gabapentin combined with Morphine is better than that by using Gabapentin or Morphine alone.

关 键 词：神经病理性疼痛 加巴喷丁 吗啡 细胞因子 

分 类 号：R745.5[医药卫生—神经病学与精神病学]