光动力对小鼠鼻咽癌移植瘤survivin表达及CD_8^+细胞毒性T细胞的影响  

The effect of photodynamic therapy on survivin expression and CD_8^+ cytotoxic T lymphocytes in nasopharyngeal carcinoma of mouse

作　　者：陈闯[1] 覃宇周[1] 谢莹[2] 欧海玲[3] 黎汉忠[1] 张作军[1] 谭志强[1] 陈蔚[1] 

机构地区：[1]广西医科大学肿瘤医院,南宁530021 [2]广西医科大学实验中心 [3]广西中医学院第一附属医院病理科

出　　处：《中国癌症防治杂志》2010年第1期5-7,共3页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西卫生厅科研基金资助课题(Z2009339)

摘　　要：目的观察氨基酮戊酸(5-Aminolevulinic acid,ALA)介导的光动力疗法(photodynamic therapy,PDT)对小鼠鼻咽癌移植瘤survivin蛋白表达及CD8+细胞毒性T细胞(CD8+ CTLs)的影响。探讨ALA-PDT治疗鼻咽癌的机制。方法32只SPF级BALB/c小鼠皮下接种鼻咽癌CNE2细胞建立荷瘤鼠模型,随机分为2组:PDT组、对照组。PDT治疗后定期采用免疫组化法检测肿瘤组织survivin蛋白表达及CD8+细胞毒性T细胞。结果治疗后24h,PDT组survivin蛋白表达明显低于对照组(P<0.05),肿瘤局部CD8+细胞数量较对照组无明显变化(P>0.05)。治疗后72h,PDT组肿瘤局部CD8+细胞数量均明显高于对照组(P<0.05)。结论抑制survivin蛋白的表达以及产生局部免疫效应均可能是ALA-PDT治疗鼻咽癌的机制之一。Objective To investigate the expression of survivin and CD＋8 cytotoxic T lymphocytes（CTL） of 5-Aminolevulinic acid（5-ALA） mediated photodynamic therapy（PDT） on mouse nasopharyngeal carcinoma（NPC）.Methods Mouse NPC model was established by subcutaneous inoculation with CNE 2 cells into the right dorsal area of BALB/c mouse.32 mice were divided into two groups at random,namely PDT group and control group.The expression of survivin and number of CTL infiltrated in tumor tissues were detected by immunohistochemical staining.Results Expression level of survivin in PDT group was significantly lower than that in control group（P0.05）.Compared to control group,the number of CTL in tumors tissue showed no significant change 24h after treatment（P0.05）.The number of CD＋8CTLs in tumor tissues of PDT group was significantly higher than that in control group 72h after treatment（P0.05）.Conclusion Suppressing the expression of survivin and immune efficacy might be one of the mechanisms for treating mouse NPC with 5-ALA mediated PDT.

关 键 词：光动力疗法 氨基酮戊酸 鼻咽癌 CD8+ CTLS survivin 

分 类 号：R739.63[医药卫生—肿瘤]