食管鳞癌中Dickkopf-3蛋白的表达  

Expression of Dickkopf-3 in esophageal squamous cell carcinoma

作　　者：张玉[1] 董卫国[1] 杨子荣[1] 雷晓斐[1] 罗和生[1] 

机构地区：[1]武汉大学人民医院消化内科,430060

出　　处：《中华内科杂志》2010年第4期325-327,共3页Chinese Journal of Internal Medicine

摘　　要：目的探讨DickkopG3（Dkk-3）在不同分化程度的食管鳞癌、正常食管黏膜组织中的表达及其与食管鳞癌生物学行为的关系。方法采用免疫组化S-P法，检测Dkk-3蛋白在67例食管鳞癌组织及5例非瘤食管组织的组织芯片中的表达情况，并结合临床病理资料进行分析。结果67例食管鳞癌组织中Dkk-3蛋白阳性率为65．7％（44／67），5例非瘤食管组织中只有1例的微血管中Dkk-3蛋白表达阳性。Dkk-3蛋白的阳性表达与食管鳞癌纤维膜浸润、淋巴结转移、浸润深度和TNM分期呈正相关（P〈0．05），而与性别、年龄、病理分级的相关性均无统计学意义（P〉0．05）。结论Dkk-3在食管鳞癌中高表达，其可能参与了食管鳞癌的浸润及转移，有助于对食管鳞癌发展的判断和预测有无淋巴结转移。Objective To investigate the expression of Dickkopf-3 （Dkk-3） in esophageal cancer and normal esophageal tissue and the relationship between Dkk-3 expression and the biological behaveior of esophageal cancer. Methods Immunohistochemical method of S-P was used to examine Dkk-3 expression in 69 cases of esophageal squamous cell carcinoma and 5 cases of normal esophageal tissue with non-tumor tissue microarray and the results were analyzed and correlated with their clinical and pathological features. Results Positive Dkk-3 expression was observed in 65.7% （44/67） of the esophageal squamous cell carcinoma cases, but only one of the five cases with normal esophageal tissue showed positive microvascular expression of Dkk-3. In cases with positive expression of Dkk-3 significant differences were found in fiber membrane infiltration, depth of invasion, lymph node metastasis and TNM staging （ P 〈 0. 05 ）, while no significant differences were found in the age, gender and pathological grading （P 〉 0. 05 ）. Conclusions The upregulation of Dkk-3 expression in esophageal squamous cell carcinoma may contribute to tumor invasion and metastasis.

关 键 词：食管肿瘤 组织芯片 Dickkopf-3 

分 类 号：R735.1[医药卫生—肿瘤]