陡脉冲电场作用于兔肝组织的剂量研究  

Study on dose of steep pulsed electric fields in rabbit liver

作　　者：凌晓娟[1] 张晨凌 熊正爱[2] 李成祥[3] 姚陈果[3] 

机构地区：[1]宝鸡市中心医院妇产科,陕西宝鸡721008 [2]重庆医科大学附属第二医院,重庆400010 [3]重庆大学高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《生物医学工程与临床》2010年第2期110-113,共4页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金资助项目(50407007)

摘　　要：目的探讨陡脉冲电场与杀伤效应的量效关系。方法将48只清洁级新西兰大白兔分为3组。电压组(21只):固定脉宽10μs、频率20Hz和时间10min,改变脉冲电压在400、500、600、700、800、900、1000V。脉宽组(12只):固定电压800V、频率20Hz和时间10min,改变脉宽为0.5、1.0、5.0、10.0μs。频率组(15只):固定电压800V、脉宽10μs和时间10min,改变频率为20、40、60、80、100Hz。结果电压组,坏死面积分别为(2.00±0.00)、(6.67±2.08)、(9.67±2.08)、(41.67±2.89)、(149.33±5.03)、(153.00±4.36)、(157.33±2.08)mm2。脉宽组,坏死面积分别为(0.00±0.00)、(6.33±1.53)、(40.67±2.52)、(149.33±5.03)mm2。频率组,坏死面积分别为(149.33±5.03)、(152.00±2.00)、(154.00±2.00)、(162.67±3.21)、(173.33±2.89)mm2。总之,陡脉冲致兔肝组织的坏死面积随着陡脉冲剂量的增大而增加。结论坏死面积与陡脉冲剂量有明显的量效关系,其值随着剂量的增大而增加。Objective To explore dose-effect relationship between the electric field therapeutic dosage and the casualty effect in rabbit liver in vivo.Methods Forty-eights rabbits were divided into 3 groups i.e.voltage group（n=21）,pulse width group（n=12） and frequency group（n=15）.The rabbit livers in vivo were exposed to steep pulsed electrical fields with serial dosage,then the rabbits were sacrificed after 72 hours,and necrosis area of livers were surveyed.Results In voltage group 21 rabbit livers in vivo were exposed to steep pulsed electrical fields with voltage of serial dosage 400 V,500 V,600 V,700 V,800 V,900 V,1 000 V,which was fixed pulse width of 10 μs,frequency of 20 Hz in 10 minutes,necrosis area of livers were（2.00 ± 0.00） mm^2,（6.67 ± 2.08） mm^2,（9.67 ± 2.08） mm^2,（41.67 ± 2.89） mm^2,（149.33 ± 5.03） mm^2,（153.00 ± 4.36） mm^2,（157.33 ± 2.08） mm^2 respectively.In pulse width group,12 rabbit livers in vivo were exposed to steep pulsed electrical fields with pulse width of serial dosage 0.5,1.0,5.0,10.0 μs ,which was fixed voltage of 800 V,frequency of 20 Hz in10 minutes,and necrosis area of livers were（0.00 ± 0.00） mm^2,（6.33 ± 1.53） mm^2,（40.67 ± 2.52） mm^2,（149.33 ± 5.03） mm^2 respectively.In frequency group,15 rabbit livers in vivo were exposed to steep pulsed electrical fields with frequency of serial dosage 20 Hz,40 Hz,60 Hz,80 Hz,100 Hz ,which was fixed voltage of 800 V,pulse width of 10 μs in 10 minutes,necrosis area of livers were（149.33 ± 5.03） mm^2,（152.00 ± 2.00） mm^2,（154.00 ± 2.00） mm^2,（162.67 ± 3.21） mm^2,（173.33 ± 2.89） mm^2 respectively.The rabbit livers in vivo were exposed to electrical pulsed fields of serial dosage and liver necrosis area increased gradually with increasing of electric-field intensity.Conclusion It is demonstrated a clear dose-effect relationship between necrotic area and steep pulsed electric fields,its value increases along with the dosage increasing.

关 键 词：陡脉冲 坏死面积 剂量学 

分 类 号：R575[医药卫生—消化系统]