污水、污泥中苏云金杆菌的分离及其基因型鉴定  

Isolation and genotype identification of Bacillus thuringiensis from waste water and sludge

作　　者：吴丽云[1,2] 何金清 周勇[1] 陈国荣 张琳[1] 张永嵘[1] 吴松清[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002 [2]湄洲湾职业技术学院,福建莆田351254 [3]福建莆田市秀屿区政府,福建莆田351146 [4]英博雪津啤酒有限公司,福建莆田351111

出　　处：《福建农林大学学报（自然科学版）》2010年第1期19-24,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家863计划(2006AA10A212);教育部博士点基金(20060389011);福建省科技创新平台建设计划(2007N2010);莆田市科技计划(2008N18)资助项目

摘　　要：从40个样品中分离到112株芽孢菌,其中有2株为Bt,占1.8%;从城市污水和啤酒厂生物处理系统中分别分离到1株Bt:采用cry1-cry11、cyt、vip3A、aiiA和inhA引物对BRC-WLY1和BRC-WLY2进行PCR扩增,发现2株Bt含有cry1(cry1Ag、cry1Ba、cry1Gb、cry1La)、cry2Ac、vip3A、aiiA杀虫基因,其中BRC-WLY1还含有inhA基因.One hundred and twelve colonies of Bacillus were isolated from 40 samples,of which 2 colonies（accounted for 1.8%） were B.thuringiensis. Among them one colony was Bt isolation,BRC-WLY1 was obtained from activated-sludge system environments from two urban sewage-digestive plants,and another Bt isolate（BRC-WLY2） was got from brewery sewage-digestive plant.The genes of cry1-cry11,cyt,vip3A,aiiA and inhA were amplified using specific primers.It showed that the two bacterial strains contained cry1（cry1Ag,cry1Ba,cry1Gb,cry1La）,cry2Ac,vip3A and aiiA genes,and BRC-WLY1 had inhA gene.

关 键 词：苏云金芽孢杆菌 啤酒污水、污泥 分离 基因鉴定 

分 类 号：S482.7[农业科学—农药学] Q786[农业科学—植物保护]