骨髓间充质干细胞在糖尿病创面中向皮肤腺上皮的分化  

Differentiation of bone marrow mesenchymal stem cells into the cells of skin appendages in diabetic wound

作　　者：钟晓红[1] 王明刚[1] 赵李平[1] 高新宇[1] 

机构地区：[1]安徽医科大学附属省立医院整形外科,安徽省合肥市230001

出　　处：《中国组织工程研究与临床康复》2010年第6期1019-1022,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：安徽省第一批重点科研项目(07021021);课题名称"骨髓间充质干细胞促进糖尿病愈合的实验";安徽高校省级自然科学研究计划项目(KJ2008B297);课题名称"人真皮多能干细胞复合血浆移植促进糖尿病创面愈合的实验研究";安徽省卫生厅医学科研课题(09A028);课题名称"肿胀麻醉技术在体表巨大肿瘤手术中的应用研究"~~

摘　　要：背景:骨髓间充质干细胞具有多向分化潜能,目前已证实其在急性创面中能够向皮肤组织细胞转化,但在糖尿病创面中的分化报道甚少。目的:观察糖尿病创面皮肤微环境中的骨髓间充质干细胞分化为皮肤附属器细胞的可行性。方法:全骨髓法分离纯化大鼠骨髓间充质干细胞,传至第三四代细胞行5-BrdU标记。采用一次性腹腔注射链脲佐菌素法建立糖尿病大鼠模型,2周后在模型鼠背部制作圆形创面,取密度为1×109L-15-BrdU标记的骨髓间充质干细胞悬液1mL注射于创面边缘,分别于细胞移植后2,3周切取创面中央再生组织制备切片,行BrdU和角蛋白免疫组织化学染色。结果与结论:BrdU阳性细胞出现在表皮、真皮及皮下组织各层,部分阳性细胞位于皮脂腺及腺导管上皮中;连续切片中BrdU阳性细胞同时也表达角蛋白,以腺导管上皮多见。提示在糖尿病溃疡创面愈合过程中,骨髓间充质干细胞具有向皮肤附属器细胞分化的潜能。BACKGROUND：Bone marrow mesenchymal stem cells （MSCs） have multi-differentiation potential.In the acute wound,MSCs have been demonstrated to have the potential for differentiating into skin cells.However,there are few reports regarding its differentiation in diabetic wound.OBJECTIVE：To observe the feasibility of differentiation of MSCs into the cells of skin appendages under the microenvironment of diabetic wound.METHODS：MSCs were isolated from the bone marrow of rats,purified and cultured.Third or fourth passage MSCs were selected and labeled with 5-bromodeoxyuridine （5-BrdU）.The rats were injected intraperitoneally with single administration of streptozocin to establish diabetes model.After 2 weeks,a round skin wound was made on the dorsal back of rats.BrdU-labeled MSCs at a density of 1×109/L were injected into the wound of the rats.The specimens were harvested from the wound tissues to prepare sections at 2 and 3 weeks after transplantation,followed by immunohistochemical staining with BrdU or keratin.RESULTS AND CONCLUSION：BrdU positive cells aggregated in the epidermis,dermis and hypodermia.Some positive cells appeared in the sebaceous glands and sebaceous duct cells and expressed keratin simultaneously in serial sections.During diabetic wound healing,MSCs have the potential to differentiate into the cells of the skin appendages.

关 键 词：糖尿病 创面愈合 皮肤附属器 细胞分化:骨髓间充质干细胞 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]