哌仑西平影响豚鼠实验性近视眼的研究  

Effect on of pirenzepine on expression of mAChRs in the ocular tissues of guinea pig with formdeprived myopia

作　　者：刘琼[1] 于健[1] 曾骏文[2] 

机构地区：[1]南方医科大学南方医院眼科,广州510515 [2]中山大学中山眼科中心眼科学国家重点实验室

出　　处：《中华眼科杂志》2010年第3期221-226,共6页Chinese Journal of Ophthalmology

摘　　要：目的探讨玻璃体腔注射选择性M，受体拮抗剂哌仑西平对豚鼠形觉剥夺性近视眼视网膜、脉络膜、巩膜和虹膜一睫状体组织中M1和M4受体mRNA表达的影响。方法采用随机分组设计的实验研究。1～2周龄的三色豚鼠24只，随机分为4组：正常对照组（N）6只，单纯形觉剥夺组（FDM）6只，药物对照组（S）6只，哌仑西平组（P）6只，均以左眼为实验眼，P组隔日玻璃体腔注射500Izg哌仑西平；S组隔日玻璃体腔注射生理盐水。21d后结束实验。提取各组眼视网膜、脉络膜、巩膜和虹膜．睫状体组织总RNA，半定量RT-PCR检测M。和M。亚型mRNA表达变化。3组间数据的比较采用单因素方差分析和Tukeyposthoe检验。结果21d时，FDM与N组相比较，FDM组眼轴相对延长0．29mm，产生相对近视-4．92D，差异有统计学意义（P〈0．001）。P组与S组相比较，眼轴相对减少了0．30mm，产生＋0．88D的相对远视，差异均有统计学意义（P〈0．001）。S组与FDM之间屈光度数的差异无统计学意义；而眼轴变化有统计学意义（s组相对延长0．08mm，而FDM组相对延长0．29mm）。半定量PCR结果显示：P组与S组相比较，其视网膜、脉络膜和虹膜睫状体组织M1和M4亚型mRNA的表达差异无统计学意义（P〉0．05）。而在后极部巩膜组织，M1和M4亚型mRNA的表达较药物对照组显著性增加（P〈0．05），其中M1受体表达增加19．16％，M4受体表达增加64．29％。结论哌仑西平能够有效抑制豚鼠形觉剥夺近视的发展。巩膜组织M1和M4亚型及其胆碱能通路可能参与M受体拮抗剂抑制近视发展。Objective To study effects of vitreous injecting M1-selective muscarinic antagonist, pirenzepine, on expression of M1 and M4 receptor in retina, choroid, sclera and iris-ciliary body of guinea pig with form-deprived myopia. Methods Twenty-four 1 -2 week-old pigmented guinea pigs were randomly divided into four groups. Groupl ： normal control （N） （ n = 6） ; group 2 ： simple form-deprived myopia （FDM） （ n = 6） ; group 3 ： drug control （ S ） （ n = 6 ） ; group 4 ： pirenzepine （P） （ n = 6 ）. Expression changes of M1 and M4 muscarinic receptors at mRNA level were detected by semi-quantitative RT-PCR in retina, choroid, sclera and iris-ciliary body. Result 1. After 21 days＇ treatment, FDM group produced relative myopia of -4. 92 D and an axial length of O. 29 mm with significance （P 〈 0. 001 ）, compared with N group. Compared with group S, the relative refractive error in group P was ＋ 0. 88 D, and axial length decreased 0. 30 mm with respectively significance （ P 〈 0. 001 ）. Differences in refractive error between group S and group FDM were not significant, but in axial length were significant （0. 08 mm vs. 0. 29 ram） （P 〈 0. 05 ）. 2. Semi-quantitative RT-PCR： M1 and M4 mRNA expression showed no significant differences in the retina, ehoroid and iris-ciliary body of group P compared with group S （ P 〉 0. 05 ）. Of interest, in the posterior selera, mRNA expression of group P was significantly greater than that of group S for the M1 （P 〈 0. 05） and M4 subtypes （P 〈0. 05）. The M1 and M4 subtype in group P was increased by ＋ 19. 16% and ＋ 64. 29% respectively. Conclusion M1-selective musearinic antagonist, pirenzepine, can effectively inhibit form-deprived myopia in guinea pig. M1 and M4 subtype in sclera and their cholinergie signaling may participate in muscarinic antagonist inhibition of myopic development.

关 键 词：哌仑西平 受体 毒蕈碱 近视 豚鼠 

分 类 号：R778.11[医药卫生—眼科]