维生素A棕榈酸酯对兔机械性角膜上皮损伤愈合及结膜杯状细胞的作用研究  

Research on effects of vitamin A palmitate on repair of mechanical corneal epithelial defects and conjunctival goblet cells in rabbits

作　　者：邱晓頔[1] 龚岚[1] 陈敏洁[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院眼科,上海200031

出　　处：《中华眼科杂志》2010年第2期151-160,共10页Chinese Journal of Ophthalmology

摘　　要：目的探讨维生素A棕榈酸酯与重组牛碱性成纤维细胞生长因子对兔机械性角膜上皮损伤愈合及结膜上皮细胞、杯状细胞的作用。方法实验研究。选取雄性新西兰大白兔120只，建立机械性角膜上皮损伤模型（角膜中央直径8mm上皮刮除），随机数字表法分为4组，每组30只。A组使用盐酸林可霉素滴眼液，B组合用维生素A棕榈酸酯凝胶与盐酸林可霉素滴眼液，C组合用重组牛碱性成纤维细胞生长因子凝胶与盐酸林可霉素滴眼液，D组合用重组牛碱性成纤维细胞生长因子凝胶与维生素A棕榈酸酯凝胶及盐酸林可霉素滴眼液。各组均于机械性角膜上皮损伤建模后当日开始用药。用法均为3次／d，1滴／次，观察时间为7d。在模型建立第0、1、4、7天共4个时间点采用前节裂隙灯显微镜照相系统进行眼表照相，并计算角膜上皮损伤及其修复面积；在模型建立前及建立后第1、4、7天共4个时间点进行角结膜组织透射电镜检查、角膜光镜组织学检查及结膜印迹细胞学检查，对角膜上皮、结膜上皮细胞修复情况及结膜杯状细胞形态数量进行分析。采用方差分析、Tukey显著性检验等对数据进行统计学分析。结果造模后第1天各组角膜上皮已愈合面积比较差异有统计学意义（F=17．663，P=0．000），角膜上皮已愈合面积A组（53．512±18．850）mm^2，B组（92．194±14．367）mm^2，C组（89．779±20．535）mm^2，D组（127．816±16．379）mm^2，B组与C组之间差异无统计学意义（P=0．995）；结膜印迹细胞学检查显示，各组结膜杯状细胞数量（每740μm×550μm面积）明显下降，A组（10．083±4．441）个，B组（10．667±3．551）个，C组（9．583±4．502）个，D组（9．167±5．606）个，但各组之间的差异无统计学意义（F=0．239，P=0．868）。造模后第4天各组角膜上皮已愈合面积比较差异有统计学意义（F=37．665，Objective Randomized controlled experimental study to investigate the influence of vitamin A palmitate and bovine recombinant basic fibroblast growth factor （bFGF） on repair of mechanical corneal epithelial defects, conjunctival epithelial ceils and goblet cells in rabbits. Methods One hundred and twenty New Zealand rabbits （ all males） were selected to establish the mechanical corneal epithelial defects models （ scratching out a round area with the diameter of 8 mm in the centre of cornea）. Forty eight New Zealand rabbits were randomly divided into 4 groups： group A used lincomycin hydrochloride eye drops （LED） after the model had been established; group B used vitamin A palmitate eye gel and LED; group C used recombinant bFGF eye gel and LED; group D used vitamin A palmitate eye gel, bFGF eye gel and LED. Photo slit lamp examination and measurement of repaired area were performed on day 0, day I, day 4 and day 7; transmission electron microscopy, histological microscope examination and impression cytology were performed on day 0, day 1, day 4 and day 7 to analysis the morphology and repairment of corneal epithelium, conjunetival epithelial cells and the goblet cells. The variants were tested using analysis of variance and Tukey＇s test. Results Statistic analysis showed that on day 1, the size of areas of repaired corneal epithelium was： group A （ 53. 512 ± 18. 850 ）mm^2, group B （ 92. 194 ± 14. 367 ） mm^2 , group C （89. 779±20. 535）mm^2, group D （127. 816 ± 16. 379）mm^2. The difference in size of repaired areas between different groups was statistically significant （ F = 17. 663, P = 0. 000 ） , with exception of the difference between groups B and C（ P = 0. 995 ）. Conjunctival impression cytology showed that, the average number of conjunctival goblet cells per 740 μm × 550 μm at day 1 was decreased, group A （ 10. 083 ± 4.441） ,group B（10. 667 ±3.551） ,group C（9. 583 ±4.502） ,group D （9. 167 ±5.606）. The difference between these f

关 键 词：上皮 角膜 伤口愈合 杯状细胞 维生素A 成纤维细胞生长因子2 

分 类 号：R772[医药卫生—眼科]