不同方法制备的蝌蚪提取液对HL-60细胞的作用  

Effects of different tadpole extracts on HL 60 cells

作　　者：杨红梅[1,2,3,4] 白蓉[1,2,3,4] 尤笑迎 丁明杰[1,2,3,4] 王黎 田爱琴[1,2,3,4] 

机构地区：[1]河南职工医学院病理生理学教研室 [2]郑州铁路局中心医院内科 [3]郑州市第五人民医院外科 [4]河南医科大学组织学与胚胎学教研室

出　　处：《河南医科大学学报》1998年第4期52-56,共5页Journal of Henan Medical University

基　　金：河南省科委科技攻关课题

摘　　要：为探讨不同方法制备的蝌蚪提取液对ＨＬ６０细胞作用的差异，利用水提法、醚提法、醇提法制备不同的蝌蚪提取液，依次称为Ｔ８７１１、Ｔ８７１２（此二者均为水提法制备，区别在于前者在水提的基础上用盐酸沉淀去除酸性蛋白）、Ｔ８７１３、Ｔ８７１４，分别将其以不同浓度与ＲＰＭＩ１６４０培养基混合，以培养ＨＬ６０细胞。结果显示：几种蝌蚪提取液对ＨＬ６０细胞的生长均有抑制作用，且在一定范围内呈浓度依赖性，其中以Ｔ８７１３的抑制效应最显著。经蝌蚪提取液作用后，Ｗｒｉｇｈｔ染色显示ＨＬ６０细胞的形态表现为向单核／巨噬样细胞分化的特征，细胞还原硝基蓝四氮唑盐（ＮＢＴ）的能力和酸性非特异性酯酶（αＮＡＥ）的活力均显著提高。提示蝌蚪提取液可抑制ＨＬ６０细胞增殖，并且诱导其向成熟的单核／巨噬样细胞分化。To investigate the different effects of the tadpole extracts prepared by different methods on HL60 cells.In the present study,the tadpole extracts prepared from tadpoles by different methods including water,ether and alcohol were called T 871 1 ,T 871 2 (the difference between both was that the former was precipitated for acid protein with HCl),T 871 3 and T 871 4 ,respectively.These extracts of different concertracions and RPMI 1640 were mixed to form terminal medium.The human promyelocytic leukemia HL 60 cells were cultured in the terminal medium.Results:The four tadpole extracts all had the antiproliferation effects on HL 60 cells in a dose dependent manner.Among the four extracts,T 871 3 was the most effective.Wright's staining showed that the extracts of tadpole induced HL 60 cells to differentiate along the monocyte macrophage pathway cytomorphologically.Twenty four hours after treatment with T 871 3 ( V/V ,1∶200),percentage of both NBT (nitro blue tetrazolium) reduction cells and α NAE (α naphthylacetate) positive cells significantly increased,compared to the control.Conclusion:These results suggest that tadpole extracts have antiproliferation effects on HL 60 cells,and also are able to induce HL 60 cells to differentiate along monocyte macrophage pathway.

关 键 词：蝌蚪 提取液 HL-60细胞系 细胞培养 抗癌药 

分 类 号：R73－351[医药卫生—肿瘤] R73－361[医药卫生—临床医学]