饲用屎肠球菌的筛选、鉴定及其生物学特性  

Screening,Identification and Characterization of Enterococcus faecium for Feed Use

作　　者：王婷婷[1] 李爱科[1] 易建明[2] 陶浩瀚[1] 汪洋[1] 

机构地区：[1]国家粮食局科学研究院,北京100037 [2]华中农业大学动物科技学院,武汉430070

出　　处：《中国粮油学报》2010年第3期89-93,97,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家科技支撑计划(2006BAD12B04);中央级公益性科研院所基本科研业务费专项课题(ZX0702)

摘　　要：分别采用肠球菌选择性培养基(EC)从健康婴儿的粪便、健康仔猪的肠道及粪便中分离筛选了4株屎肠球菌,分别命名为E fB、E f1-2、E f3-4、E f4-1,利用B iolog自动微生物分析系统鉴定结果表明,它们都是屎肠球菌,而且经16S rRNA基因序列测序比较分析,表明这4株菌与数据库中已发表的屎肠球菌16S rRNA序列(AccessionNo.AY675247)同源性均为99%,两者鉴定结果一致。试验表明,所筛得菌株生长速度较快,且对致病型大肠杆菌K88,K99,金黄色葡萄球菌,巴氏杆菌及沙门氏菌都有较强的抑制作用,其中对大肠杆菌K99的抑菌效果最好。屎肠球菌新菌种的鉴定与筛选为开发新型微生态类饲料添加剂提供了帮助。Four new strains of Enterococcus faecium were screened from healthy infant feces,piglet intestine and feces in EC medium,which were named as EfB,Ef1-2,Ef3-4 and Ef4-1.They were all identified as Enterococcus faecium by Biolog automatic microbe analysis system.16S rRNA identification comparing with GenBank indicated that there was 99% homology with Enterococcus faecium strain SF（Accession No.AY675247）.Results：All the four new strains have faster growth rate and can significantly inhibit growth of Escherichia coli K88,Escherichia coli K99,Staphylococcus Aureus,Pasteurellosis Bacillus and Salmonella,especially Escherichia coli K99.The identification results with two ways are consistent.The screening,identification and development of new Enterococcus faecium would be beneficial for the development of micro-ecological agents for feed use.

关 键 词：屎肠球菌 Biolog自动微生物分析系统 16S rRNA鉴定 生长代谢曲线 抑菌能力 

分 类 号：Q93-331[生物学—微生物学]