高迁移率族蛋白A1(HMGA1)在肝细胞癌中的表达及临床意义  

The expression and clinical significance of HMGA1 in hepatocelluar carcinoma

作　　者：许永华[1] 李长贤[1] 陈杰[1] 姚爱华[1] 李相成[1] 

机构地区：[1]南京医科大学第一附属医院肝移植中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第3期304-308,365,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省"科教兴卫工程"医学重点人才基金(RC2007056)

摘　　要：目的:研究肝细胞癌(hepatocelluar carcinoma,HCC)中高迁移率族蛋白A1(high mobility group protein A1,HMGA1)的表达情况,探讨HMGA1基因在HCC中的表达与肝内转移以及临床病理参数之间的关系。方法:采用实时定量荧光聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,QRT-PCR)检测52例肝细胞癌组织和配对的癌旁组织、10例正常肝脏组织中HMGA1mRNA的表达,用免疫组化法(immunohistochemistry,IHC)检测HMGA1蛋白在肝细胞癌中的表达,评价HMGA1的表达与HCC临床病理学因素的相关性。结果:肝癌组织中HMGA1mRNA的表达较正常肝脏中升高6.82倍,其中有、无肝内转移组较正常肝组织分别升高13.36倍和4.63倍(P<0.05)。HMGA1mRNA的表达与TNM分期、Edmonson分级、肝内转移有关(P<0.05)。肝细胞癌中有肝内转移组较无肝内转移组HMGA1蛋白表达明显增强。结论:HMGA1基因在肝细胞癌中的表达较正常肝脏组织中明显升高,并与肿瘤的肝内转移相关,有望成为肝细胞癌诊断和治疗的新靶点。Objective：To detect the expression of high mobility group protein A1（HMGA1） in HCC and investigate the relationship between the expression of HMGA1 and clinic pathological features of HCC. Methods：QRT-PCR was performed for detecting the expression of HMGA1 mRNA in 52 HCC tumor specimen and corresponding paracancerous liver tissues,10 normal human liver cancer tissue was used as negative control. The expression of HMGA1 protein in HCC was analyzed by immunohistochemistry. Results：The expression of HMGA1 mRNA was 6.82-fold higher in HCC tissues than in normal liver tissues （P〈0.05）. And in HCC tissues with and without intrahepatic metastasis,the HMGA1 mRNA expression was 13.36 and 4.63 folds of that in normal liver tissues,respectively. The expression level of HMGA1 in HCC tissues was related to TNM stage,Edmonson grade and intraheptic metastasis（P〈0.05）. The expression of HMGA1 protein was significantly higher in HCC with intrahepatic metastasis than in that without intrahepatic metastasis. Conclusion：HMGA1 expression was up-regulated in HCC,and it might be associated with the clinic stages and intrahepatic metastasis of HCC,and potentially used as an indicator for predicting the prognosis and a target for treatment approach.

关 键 词：肝细胞癌 HMGA1 肝内转移 实时定量PCR 

分 类 号：R735.7[医药卫生—肿瘤]