粟谷发芽期间贮藏物质动员及γ-氨基丁酸富集技术  

Study on Mobilization of Stored Reserves and Accumulation of γ-aminobutyric Acid in Foxtail Millet During Germination

作　　者：白青云[1,2] 曾波[2] 顾振新[2] 

机构地区：[1]淮阴工学院生化学院食品工程系,江苏淮安223003 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品与发酵工业》2010年第3期65-69,共5页Food and Fermentation Industries

基　　金：江苏省高新技术项目(BG2004323)

摘　　要：以5种粟谷(JG-34、JG-36、77-322、JG-41和SG-1)为试材,在25℃黑暗条件下发芽60h,探讨粟谷发芽期间贮藏物质的动员和γ-氨基丁酸(GABA)含量的变化。结果表明:60h发芽期间,5种粟谷的呼吸强度呈现增长趋势,其中SG-1和JG-34的呼吸强度最高;淀粉含量降低幅度为43%-60%;还原糖和游离氨基酸含量分别增加60%-136%和127%-180%,其中以JG-34中游离氨基酸增量最多;可溶性蛋白含量呈增加趋势。发芽期间粟谷GABA含量得到有效增长,JG-34、77-322、SG-1、JG-41和JG-36中GABA含量分别比发芽前提高2.41、2.23、1.92、2.18和2.61倍,其中JG-34发芽后GABA含量最高,达到135.4μg/g。通过对不同品种粟谷发芽期间GABA富集量的差异分析得出,JG-34是最适合富集GABA的粟谷品种。粟谷发芽期间富集的GABA含量与其芽长、呼吸强度和游离氨基酸含量呈显著正相关。Mobilization of stored reserves and γ-aminobutyric acid （GABA） during a 60 h germination of five foxtail millet cultivars （JG-34, JG - 36, 77 - 322, JG - 41 and SG - 1 ） were investigated in this study. The millet were germinated at 25℃ in dark incubator. The results showed that during 60 h of germination, respiration rate in five cultivars increased, SG - 1 and JG - 34 were higher than other cultivars ; starch content decreased significantly in the range of 43% -60 % ; reducing sugar content and free amino acid content increased in the range of 60% -136 % and 127% -180 % , respectively; soluble protein content increased during all germination periods. There are significant increase of GABA content in five millet cultivars during germination, the increased extent were 1.92 - 2.61 - fold, GABA content in JG - 34 had the highest among all cuhivars tested, which lead to 13.54 mg/100g. Variance a- nalysis indicated that there were significant difference （P 〈 0.01 ） in GABA content among all cuhivars. JG - 34 of five cultivars could be considered as a good source for GABA accumulation. GABA content in millet during germination was significantly positive correlation with sprout length, respiration rate, soluble protein and free amino acid, respectively.

关 键 词：粟谷 发芽 贮藏物动员 GABA富集 

分 类 号：S515[农业科学—作物学]