反义核酸药物癌泰得对乳腺癌细胞株的体外杀伤作用  

In vitro killing effect of cantide on breast cancer cell strains

作　　者：张帆[1] 冯帆[2] 任磊[2] 谢文秀[1] 李自健[1] 杨俊兰[1] 

机构地区：[1]解放军总医院肿瘤中心,北京100853 [2]军事医学科学院,北京100850

出　　处：《军医进修学院学报》2010年第4期370-371,共2页Academic Journal of Pla Postgraduate Medical School

基　　金：罗氏基金合作项目(03YH606)

摘　　要：目的探讨反义核酸药物癌泰得对乳腺癌细胞株的体外杀伤作用。方法1)MTT法测定不同浓度癌泰得对乳腺癌细胞株BT474的抑制率。2)Annexin/PI双染法,流式细胞仪检测细胞凋亡比率。结果MTT法观察到癌泰得对BT474细胞的杀伤作用,流式细胞仪检测到BT474细胞凋亡率达16.82%,以晚期凋亡为主。结论癌泰得对BT474细胞有抑制作用,抑制率与浓度呈线性相关。Objective To investigate the in vitro killing effect of an antisense oligodeoxynucleotide drug, cantide, on breast cancer cell strains. Methods Inhibition rate of cantide for BT474 breast caner cell strains was assayed by MTT and cell apoptosis rate was detected by flow cytometry with Annexin/PI double staining. Results MTT showed the killing effect of cantide on BT474 cells.After Annexin/PI staining, flow cytometry showed that the apoptosis rate of BT474 cells was 16.82%, mainly manifested as end-stage apoptosis. Conclusion Cantide has a strong inhibitory effect on breast cancer BT474 cells in a concentration-dependent manner.

关 键 词：反义寡核苷酸 乳腺肿瘤 端粒 末端转移酶 

分 类 号：R963[医药卫生—微生物与生化药学]