过氧化氢存在下极谱催化波法测定桑枝中桑色素的含量  

Determination of Morin in Morus Alba L.by Polarographic Catalytic Wave in the Presence of H_2O_2

作　　者：程司堃[1] 王晶[2] 李健[1] 黄国海[1] 张晓楠[3] 罗二平[2] 

机构地区：[1]第四军医大学药学系化学教研室,西安710032 [2]第四军医大学生物医学工程系卫生装备教研室,西安710032 [3]第四军医大学药学系药理教研室,西安710032

出　　处：《医药导报》2010年第4期519-522,共4页Herald of Medicine

基　　金：陕西省科技攻关计划(基金编号:2009K13-01-19)

摘　　要：目的考察过氧化氢(H2O2)存在下桑色素的极谱催化波,建立高灵敏度测定桑枝中桑色素含量的方法。方法采用电分析化学法。结果在0.2mol·L-1醋酸-醋酸钠(pH值5.3±0.1)和2.0×10-2mol.L-1H2O2最佳介质中,桑色素极谱催化波峰电流与其浓度在6.0×10-8～3.0×10-6mol.L-1范围内有线性关系。结论该方法灵敏度高、快速、简便,从电化学角度为桑色素清除氧自由基提供实验依据。Objective Polarographic catalytic wave of morin in the presence of H2O2 was investigated,by which morin was determined in Morus Alba L.sensitively.Methods The determination was carried out by electroanalytical chemistry.Results In 0.2 mol·L^-1 HAc-NaAc（pH 5.3±0.1）and 2.0×10^-2 mol·L^-1 H2O2 supporting electrolyte,the peak current was linearly proportional to morin＇s concentration in the range from 6.0×10^-8 mol·L^-1 to 3.0×10^-6 mol·L^-1.Conclusion Compared with that of reported,this method is characterized as sensitivity,rapidness and convenience.Furthermore,the investigation of catalytic wave of morin is helpful in understanding its oxygen free radical scavenging ability.

关 键 词：桑色素 过氧化氢 极谱催化波 

分 类 号：R282.71[医药卫生—中药学] O657.14[医药卫生—中医学]