鹰嘴豆EST资源中SSR的信息分析  

Information analysis of EST resource of chickpea

作　　者：王显生[1] 贾钰莹[1] 顾汉艳[1] 何小玲[1] 张桦[2] 张巨松[2] 石书兵[2] 李建贵[2] 麻浩[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]新疆农业大学农业生物技术重点实验室,新疆乌鲁木齐830052

出　　处：《湖南农业大学学报（自然科学版）》2010年第2期137-141,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家科技支撑计划项目(2006BAD09A04;2006BAD09A08;2007BAC15B06);国家自然科学基金项目(30860152);国家博士后科学基金项目(20080431107);高等学校学科创新引智计划项目(教育部);科技支疆项目(200991254)

摘　　要：从NCBI公共数据库下载获得27904条鹰嘴豆表达序列标签EST,去除其中低质量的和冗余序列后得到全长为6198092bp的11224条无冗余EST.利用SSRIT(Simple sequence repeat identification tool)软件共在这些序列中发掘出982个SSR,它们分布于865条EST中,出现频率为8.69%,平均长度为15.58bp,平均距离为6.31kb.在鹰嘴豆EST-SSR中,二核苷酸重复是最主要的重复类型(66.90%),其次是三核苷酸重复类型(30.75%),出现最多的重复基元类型是TA/AT(24.95%).27 904 ESTs of chickpea （Cicer arietinum L.） were downloaded from the database of NCBI and some redundant or low quality sequences were removed. Finally, 11 224 non-redundant ESTs with total length of 6 198 092 bp were obtained. SSRIT （Simple sequence repeat identification tool）, a web tool for SSR hunting, was used to analyze the sequences. The results showed that a total of 982 SSRs distributed in 865 ESTs were detected, with the frequency for the non-redundant ESTs being 8.69%. The average length and distribution distance of the EST-SSRs were about 15.58 bp and 6.31 kb. Among the different SSRs ranging from 2 to 7 bp, dinucleotide repeats were most abundant （66.90%）, followed by trinucleotide repeats （30.75%）. Among 83 identified motif types, TA/AT had the highest EST frequency （24.95%）. The results would contribute to the development of EST-SSR markers in chickpea.

关 键 词：鹰嘴豆 表达序列标签-简单重复序列 频率 特性 

分 类 号：S529.024[农业科学—作物学]