大蒜中多种农药残留测定的气相色谱-质谱联用技术研究  

Determination of Multiple Pesticides Residue in Garlic by Gas Chromatography/Mass Spectrometry

作　　者：郭永泽[1] 张玉婷[1] 刘磊[1] 邵辉[1] 李辉[1] 李娜[1] 

机构地区：[1]天津市农业科学院中心实验室,天津300381

出　　处：《安徽农业科学》2010年第8期3870-3873,共4页Journal of Anhui Agricultural Sciences

基　　金："重大新药创制"科技重大专项课题(2009ZX09502-027);天津市应用基础及前沿技术研究计划(07JC2DJC03500);天津市农业科技成果转化与推广项目(0803110)

摘　　要：[目的]建立同时检测大蒜中211种农药残留的气相色谱-质谱联用技术。[方法]大蒜经微波使酶失活后,用乙腈和水溶液匀质提取,PSA和C18固相萃取柱净化,用气相色谱-质谱联用仪进行测定。[结果]各农药的方法最低检出浓度(LOD)在0.015～0.082mg/kg,大部分农药品种的方法最低检出浓度低于0.020mg/kg。在加标水平为0.1mg/kg时,相对标准偏差均不超过15%,仅有个别农药回收率低于70%。[结论]该研究为去除含硫蔬菜中农药残留测定时硫化物的干扰提供了新的途径。[Objective] The research aimed to establish gas chromatography/mass spectrometry （GC/MS） and determine 211 kinds of pesticides residues in garlic.[Method] Garlic was firstly treated with microwave to inactivate the enzymes.Acetonitrile and water were used for homogenized exrraction.And PSA and C18 solid-phase extraction （SPE） column were used for purification.And pesticide residues were determined by using GC/MS.[Result] The limit of detection （LOD） of all pesticides was in a range of 0.015-0.082 mg/kg and most LOD of pesticides were below 0.020 mg/kg.When the standard injection level was 0.1 mg/kg,all relative standard deviations were less than 15%.Only the individual pesticides had recoveries below 70%.[Conclusion] The research provided new approaches for removing the disturbance of sulphide in the determination of S-contained vegetables.

关 键 词：大蒜 微波处理 农药残留 GC/MS 

分 类 号：S633.4[农业科学—蔬菜学]